Goat anti-Bovine IgG (H&L), ALP conjugated

AS16 3246 | Clonality: Polyclonal | Host: Goat | Reactivity: Bovine IgG (H&L)


product information
Background		Goat anti-Bovine IgG (H&L), is a secondary antibody, conjugated to ALP, which binds to Bovine IgG (H&L) in immunological assays.
Immunogen		Purified Bovine IgG, whole molecule
Host		Goat
Clonality		Polyclonal
Clone
Purity		Affinity purified
Format		Liquid
Quantity		1.0 mg
Reconstitution
Storage		Store non-diluted antibody at Store at 2-8°C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Tested applications		ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related products		other anti-bovine, ALP conjugated, secondary antibodies
Additional information		Antibody purity is > 95% based on SDS-PAGE. Antibody concentration is 1.5 mg/ml (E 1% at 280 nm = 13.0). Affinity purified using solid phase Bovine IgG (H&L). Antibody is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as preservative. Based on IEP, this antibody reacts with: · heavy (γ) chains on bovine IgG · light chains on all bovine immunoglobulins. Based on IEP, no reactivity is observed to: · non-immunoglobulin bovine serum immunoglobulins.

application information
Recommended dilution		1 : 50 000-1 : 5 000 (ELISA), 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Expected \| apparent MW
Confirmed reactivity		Bovine IgG (H&L)
Predicted reactivity
Not reactive in
Additional information
Selected references		To be added when available, antibody released in March 2016.

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