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Goat anti-Bovine IgG (H&L), ALP conjugated

AS16 3246 | Clonality: Polyclonal | Host: Goat | Reactivity: Bovine IgG (H&L)
Goat anti-Bovine IgG (H&L), ALP conjugated in the group Secondary Antibodies / Anti-Bovine  / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS16 3246)

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Product Information

Immunogen Purified Bovine IgG, whole molecule
Host Goat
Clonality Polyclonal
Purity Affinity purified
Format Liquid
Quantity 1.0 mg
Storage Store non-diluted antibody at Store at 2-8°C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 50 000-1 : 5 000 (ELISA), 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)

Reactivity

Confirmed reactivity Bovine IgG (H&L)

Application examples

Additional information

Additional information Antibody purity is > 95% based on SDS-PAGE.
Antibody concentration is 1.5 mg/ml (E 1% at 280 nm = 13.0).
Affinity purified using solid phase Bovine IgG (H&L).
Antibody is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as preservative.

Based on IEP, this antibody reacts with: · heavy (γ) chains on bovine IgG · light chains on all bovine immunoglobulins.
Based on IEP, no reactivity is observed to: · non-immunoglobulin bovine serum immunoglobulins.

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Background

Background Goat anti-Bovine IgG (H&L), is a secondary antibody, conjugated to ALP, which binds to Bovine IgG (H&L) in immunological assays.

Product citations

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