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Goat anti-Donkey IgG (H&L), HRP conjugated

AS16 3281 | Clonality: Polyclonal | Host: Goat | Reactivity: Donkey IgG (H&L)

Goat anti-Donkey IgG (H&L), HRP conjugated in the group Secondary Antibodies / Anti-Donkey at Agrisera AB (Antibodies for research) (AS16 3281)

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Product Information

Immunogen Purified Donkey IgG, whole molecule.
Host Goat
Clonality Polyclonal
Purity Affinity purified using solid phase donkey IgG.
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Storage Store freeze-dried powder at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Tested applications Flow cytometry (Flow cyt), Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 20-1 : 2000 (Flow cyt), (IL), 1: 5000 (WB)

Reactivity

Confirmed reactivity Heavy chains on donkey IgG and light chains on all donkey immunoglobulins.
Not reactive in Non-immunoglobulin donkey serum proteins.

Application examples

Additional information

Additional information

Antibody purity is > 95% based on SDS-PAGE.

Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.

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Background

Background Goat anti-Donkey IgG (H&L) is a secondary antibody, conjugated to HRP, which binds to Donkey IgG (H&L) in immunological assays.

Product citations

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