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Goat anti-Human IgG Fc, F(ab)'2 Fragment, DyLightŪ 488 conjugated, min. cross-reactivity to human IgA, IgM or IgG F(ab)'2 fragment

AS16 3353 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG Fc, F(ab)'2 Fragment 
Goat anti-Human IgG Fc, F(ab)'2 Fragment, DyLightŪ 488 conjugated, min. cross-reactivity to human IgA, IgM or IgG F(ab)'2 fragment in the group Secondary Antibodies / Anti-Human / Fluorescent / IgG at Agrisera AB (Antibodies for research) (AS16 3353)

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Product Information

Immunogen Purified Human IgG, Fc fragment
Host Goat
Clonality Polyclonal
Purity Affinity purified
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution add 0.55 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store freeze-dried powder at 2-8 °C.
Tested applications Flow cytometry (Flow cyt), Immunolocalization (IL)
Recommended dilution 1 : 20-1 : 2000 (Flow cyt), (IL)

Reactivity

Confirmed reactivity Goat anti-Human IgG Fc, F(ab)'2 Fragment.

Application examples

Additional information

Additional information

Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Affinity purified using solid phase Human IgG .
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.

Based on IEP, this antibody reacts with: • heavy (γ) chains on human IgG .
Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins • light chains on all human immunoglobulins • human IgA or IgM .

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Background

Background

Goat anti-Human IgG Fc, F(ab)'2 Fragment, DyLight® 488 conjugated, min. cross-reactivity to human IgA, IgM or IgG F(ab)'2 fragment  is a secondary antibody conjugated to DyLight® 488, which binds to human IgG Fc, F(ab)'2 Fragment in immunological assays.
DyLight® 488 has Amax = 493 nm, Emax = 518 nm.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

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