Purified pools of homogenous IgA isolated from pooled rat serum,
Purified by salt precipitation, followed by a ion-exchange chromatography with DEAE Sepharose, in PBS pH 7.2.
For reconstitution add 1 ml of sterile destilled water, Spin down to remove insoluble particles
Store at +4°C, for long time storage, store at -20°C.
ELISA (ELISA), Immunocytochemistry (ICC), Immunofluorescence (IF) Immunohistochemistry (frozen) (IHC)
Has to be determined for each specific application