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H3K27me3 | Histone H3, trimethylated lysine 27 (H3K27me3)

AS16 3193 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, C. elegans, Human, Mouse, Populus sp. , S. lycopersicum,  Z. mays
H3K27me3 | Histone H3, trimethylated lysine 27 (H3K27me3) in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3193)
H3K27me3 | Histone H3, trimethylated lysine 27 (H3K27me3)



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Product Information

Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum.
Format Liquid
Quantity 50 ĩg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
Recommended dilution 0,5-1 ĩg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 20 000 (PA), 1 : 1000 (WB)
Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Arabidopsis thaliana, Caenorhabditis elegans, Mouse, Populus sp. , Solanum lycopersicum,  Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using anti-H3K27me3 | H3, trimethylated lysine 27 (H3K27me3)  polyclonal antibdies


Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using anti-H3K27me3 antibodies. The antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.

Additional information

Additional information Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300

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Background

Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K27 is associated with inactive genomic regions.

Product citations

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