H3K27me3 | Histone H3, trimethylated lysine 27 (H3K27me3)
AS16 3193 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, C. elegans, Human, Mouse, Populus sp. , S. lycopersicum, Z. mays

Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum.
Format
Liquid
Quantity
50 µg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
Recommended dilution
0,5-1 µg/IP (ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 20 000 (PA), 1 : 1000 (WB)
Expected | apparent MW
15 kDa
Reactivity
Confirmed reactivity
Human
Predicted reactivity
Arabidopsis thaliana, Caenorhabditis elegans, Mouse, Populus sp. , Solanum lycopersicum, Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using anti-H3K27me3 antibodies. The antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.

Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using anti-H3K27me3 antibodies. The antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.
Additional information
Additional information
Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300
Background
Background
Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K27 is associated with inactive genomic regions.
Product citations
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