H3K27me3 | Histone H3, trimethylated lysine 27 (H3K27me3)
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Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using anti-H3K27me3 antibodies. The antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.
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