HSF1 | Heat shock factor 1
AS06 178 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomons reinhardtii
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Product Information
Immunogen
C-terminal part of Chlamydomonas reinhardtii HSF1 (Exons 7 to 9), UniProt: A1YTN3
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 ĩl
Reconstitution
For reconstitution add 100 ĩl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1. 3000 (WB)
Expected | apparent MW
78.4 | 100 kDa
Reactivity
Confirmed reactivity
Chlamydomonas reinhardtii
Predicted reactivity
Chlamydomonas reinhardtii
Not reactive in
Higher plants
Application examples
Additional information
Unspecific cross-reactions at 40-50 kDa.
Background
Background
HSF1 is a key regulator of the stress response. It becomes hyperphosphorylated following heat shock (results in the shifting of the HSF1 protein to slightly higher MW) and HSF1 protein rapidly accumulates during heat stress. HSF1 appears to be constitutively present as high molecular weight complexes, presumably trimers. It also interacts with cytosolic HSP70A.
Product citations
Selected references
Schulz-Raffelt et al. (2007).. Heat shock factor 1 is a key regulator of the stress response in Chlamydomonas. The Plant Journal 52: 286-295
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