LYC | Lycopene beta cyclase (chloroplastic)

AS13 2709 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. annuum, O. sativa, P. sativum, H.pluvialis (green alga)

Product Information

Immunogen

His-tagged recombinant part of Arabidopsis thaliana lycopene beta-cyclase, encompassing about 2/3 of the coding region of, AEE74875.1 ,TAIR: AT3G10230

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 500-1 : 2000 (WB)

Expected | apparent MW

56 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Capsicum annuum, Pisum sativum, Haematococcus pluvialis (green alga), Oryza sativa

Predicted reactivity
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Not reactive in diatoms

Application examples

Additional information

Related products

AS13 2710 | Anti-IPP isomerase | isopentyl pyrophosphate isomerase, rabbit antibodies

AS15 3079 | Anti-LCY | lycopene beta-cyclase (chloroplastic), rabbit antibodies (to full length LCY)

other antibodies to carotenoid biosynthesis pathway

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background Lycopene cyclase (EC=5.5.1.19) is an enzyme involved in carotenoid biosynthesis, which catalyzes the double cyclization reaction which converts lycopene to beta-carotene and neurosporene to beta-zeacarotene. Synonymes:

Product citations

Selected references Tang el al. (2020). OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell. 2020 May 4;53(3):272-286.e7. doi: 10.1016/j.devcel.2020.03.009.
Sun et al. (1998). Differential expression of two isopentenyl pyrophosphate isomerases and enhanced carotenoid accumulation in a unicellular chlorophyte. PNAS, Vol. 95, pp. 11482–11488, September 1998.