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Non-fucosylated xyloglucan-1 (clone CCRC-M101)

AS16 3230-1ml  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana, Solanum lycopersicum

From the laboratory of Michael G. Hahn, PhD, University of Georgia

Non-fucosylated xyloglucan-1 (clone CCRC-M101) in the group Antibodies Plant/Algal  / Cell Wall / Dr. Hahn collection, University of Georgia at Agrisera AB (Antibodies for research) (AS16 3230-1ml)
Non-fucosylated xyloglucan-1 (clone CCRC-M101)



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Product Information

Immunogen BSA-conjugated tomato (Lycopersicum esculentum) xyloglucan, covalent binding.
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution Undiluted or at 1 : 10 (ELISA), (IF), (IHC)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Solanum lycopersicum
Predicted reactivity Dicots
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Exact working dilution needs to be determined by end user
CCRC-M101 does not bind to XXXG, but does bind to other xyloglucan oligosaccharides, CCRC-M101 also binds to pectic polysaccharide preparations from several plants

Related products

Background

Background Xyloglucans are polysaccharides commonly refered to as hemicelluloses found in the primary cell walls of vascular plants. This antibody recognizes the glycan group of xyloglucan-1 in the non-fucosylated form.

Product citations

Selected references Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
Confirmed reactivity: Arabidopsis thaliana, Solanum lycopersicum
predicted reactivity: Dicots
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Clonality: Monoclonal
Format: Liquid
Host: Mouse
immunogen: BSA-conjugated tomato (Lycopersicum esculentum) xyloglucan, covalent binding.
Purity: Cell culture supernatant.
Quantity: 1 ml
recommended dilution: Undiluted or at 1 : 10 (ELISA), (IF), (IHC)
storage: Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class: IgG1
tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
All references: Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
additional information: Exact working dilution needs to be determined by end user
additional information (application): CCRC-M101 does not bind to XXXG, but does bind to other xyloglucan oligosaccharides, CCRC-M101 also binds to pectic polysaccharide preparations from several plants
background: Xyloglucans are polysaccharides commonly refered to as hemicelluloses found in the primary cell walls of vascular plants. This antibody recognizes the glycan group of xyloglucan-1 in the non-fucosylated form.