OLE9 | Major pollen allergen Ole e 9
AS19 4348 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Olea europaea
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Varied amounts of recombinant OLE9 protein were loaded/well and separated on 10 % SDS-PAGE and blotted 1h to PVDF (0.45 µm) using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 2.8 µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions.
Alternative names: Glucan endo-1,3-beta-D-glucosidase (Major pollen allergen Ole e 9) (allergen Ole e 9), OLE9
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