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OLE9 | Major pollen allergen Ole e 9

AS19 4348 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Olea europaea

OLE9 | Major pollen allergen Ole e 9 in the group Antibodies for Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS19 4348)

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Product Information

Immunogen Recombinant Olea europaea Glucan endo-1,3-beta-D-glucosidase protein, amino acids: 360-460, UniProt: Q94G86

Host Rabbit
Clonality Polyclonal
Purity >95%, Protein G purified, total immunoglobulin G fraction
Format Liquid
Quantity 50 µg
Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 48.8 kDa

Reactivity

Confirmed reactivity Olea europaea

Application examples

Application examples application example



Western blot using anti-OLE9 rabbit antibodies

Varied amounts of recombinant OLE9 protein were loaded/well and separated on 10 % SDS-PAGE  and blotted 1h to PVDF (0.45 µm) using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 2.8 µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions. 

Additional information

Additional information Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4

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Background

Background OLE9 | Major pollen allergen Ole e 9 is an enzyme (EC 3.2.1.39), which is catalyzing Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Alternative names:
Glucan endo-1,3-beta-D-glucosidase  (Major pollen allergen Ole e 9) (allergen Ole e 9), OLE9

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