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OLE9 | Major pollen allergen Ole e 9
AS19 4348 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Olea europaea
- Product Info
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Immunogen: Recombinant Olea europaea Glucan endo-1,3-beta-D-glucosidase protein, amino acids: 360-460, UniProt: Q94G86
Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein G purified. Format: Liquid Quantity: 50 µg Storage: Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 48,8 kDa - Reactivity
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Confirmed reactivity: Olea europaea - Application Examples
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application example
Varied amounts of recombinant OLE9 protein were loaded/well and separated on 10 % SDS-PAGE and blotted 1h to PVDF (0.45 µm) using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 2.8 µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions.
- Additional Information
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Additional information: Preservative: 0,03% Proclin 300, Preparation contains: 50% Glycerol, 10 mM PBS, pH 7,4 - Background
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Background: OLE9 | Major pollen allergen Ole e 9 is an enzyme (EC 3.2.1.39), which is catalyzing Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Alternative names: Glucan endo-1,3-beta-D-glucosidase (Major pollen allergen Ole e 9) (allergen Ole e 9), OLE9 - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
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10 % until end of 2023. Use discount code: Conj10
AS15 2892 | clonality: polyclonal | host: rabbit | reactivity: Olea europea