Anti-PDLP1 | Plasmodesmata-located protein 1

Product no: AS21 4610

AS21 4610   | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PDLP1 protein, UniProt: Q8GXV7, TAIR: AT5G43980
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 32.6  | 35 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Species of your interest not listed? Contact us
    Not reactive in: Populus sp.
  • Application Examples
  • Western blot using anti-PDLP1 antibodies

    17 ug/ul of proteins extracted freshly from purified plasmodesmata fraction from Arabidopsis thaliana liquid cell culture were denatured with Laemmli buffer at 50°C for 30 minutes. Separated on 12 % SDS-PAGE and blotted to nitrocellulose using semi-dry transfer. Blot blocked with 5% milk for 1h/RT. Rinsed once with TBS-0.1% Tween and incubated O/N with primary antibody in 5% BSA TBS-0.1% Tween. Rinsed 6 x/ 5 min in TBS-0.1% Tween and incubated with secondary (anti-rabbit IgG horse radish peroxidase) diluted 1 : 10 000/1h. Rinsed 6 x/ 5 min in TBS-0.1% Tween with last wash being TBS only. Incubated with chemiluminescent reagent, according to manufacture's instructions. Exposure time: 60 seconds.

    Courtesy of Dr. Emmanuelle Bayer, Laboratory of Membrane Biogenesis, CNRS- Université Bordeaux, France

    Western blot using anti-PDLP1 antibodies

    Each lane contains 20 µg of Arabidopsis thaliana protein. Cotyledons and hypocotyls of seedlings were homogenized and incubated with a detergent solution (sodium deoxycholate) for different amounts of time. After centrifugation proteins were extracted from the pellet with 8M UTU (6M Urea and 2M Thiourea) or precipitated from the supernatant with Acetone.

    20 µg/well of total protein extracted freshly from hypocotyls and cotyledons of seedlings.  Exact buffer components were: 100 mM Tris-HCL pH 8, 150 mM NaCl, 10 mM EDTA, 1 mM DTT, 10% Glycerol, 2 mM Na-Deoxycholate, protease inhibitor cocktail. Proteins were denatured with 70 °C 10 min.  Samples were separated in 12% SDS-PAGE and blotted for 1h to nitrocellulose, using: semi-dry transfer. Blot was blocked with Roche Western Blotting Reagent (11921681001) for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:  5000 with agitation in TBS-T ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody diluted to 1: 25 000 in  for 1h/RT with agitation (AS09 602). The blot was washed as above and developed with a following chemiluminescent detection reagent: AS16 ECL-N-10. Exposure time was ~15 minutes.

    Courtesy of Dr. Sven Gombos, Universität Hohenheim, Stuttgart, Germany

  • Background
  • Background: PDLP1 (Plasmodesmata-located protein 1) mediates callose deposit during fungal infection and is required for systemic acquired resistance (SAR), mediated by azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Alternative names: PD-located protein 1,Cysteine-rich repeat secretory protein 56, Plasmodesmata localizing protein 1.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
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