PDLP1 | Plasmodesmata-located protein 1

Product no: AS21 4610

AS21 4610   | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PDLP1 protein, UniProt: Q8GXV7, TAIR: AT5G43980
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 32.6  | 35 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Species of your interest not listed? Contact us
    Not reactive in: Populus sp.
  • Application Examples
  • Western blot using anti-PDLP1 antibodies

    17 ug/ul of proteins extracted freshly from purified plasmodesmata fraction from Arabidopsis thaliana liquid cell culture were denatured with Laemmli buffer at 50°C for 30 minutes. Separated on 12 % SDS-PAGE and blotted to nitrocellulose using semi-dry transfer. Blot blocked with 5% milk for 1h/RT. Rinsed once with TBS-0.1% Tween and incubated O/N with primary antibody in 5% BSA TBS-0.1% Tween. Rinsed 6 x/ 5 min in TBS-0.1% Tween and incubated with secondary (anti-rabbit IgG horse radish peroxidase) diluted 1 : 10 000/1h. Rinsed 6 x/ 5 min in TBS-0.1% Tween with last wash being TBS only. Incubated with chemiluminescent reagent, according to manufacture's instructions. Exposure time: 60 seconds.

    Courtesy of Dr. Emmanuelle Bayer,Laboratory of Membrane Biogenesis, CNRS- Université Bordeaux, France

  • Background
  • Background: PDLP1 (Plasmodesmata-located protein 1) mediates callose deposit during fungal infection and is required for systemic acquired resistance (SAR), mediated by azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Alternative names: PD-located protein 1,Cysteine-rich repeat secretory protein 56, Plasmodesmata localizing protein 1.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
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