Name:
Phone:
E-mail:
Address:






PBP1 | PYK10-binding protein 1 (C-terminal)

AS20 4413 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana
PBP1 | PYK10-binding protein 1 (C-terminal) in the group Antibodies Plant/Algal  / Environmental Stress / Pathogen attack at Agrisera AB (Antibodies for research) (AS20 4413)
PBP1 | PYK10-binding protein 1 (C-terminal)



DATA SHEET IN PDF

Qty: 
349
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Conjugated peptide, derived from C-terminus of Arabidopsis thaliana PBP1, UniProt: O04314, TAIR: At3g16420
Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format Liquid at 2 mg/ml.
Quantity 200 ĩg
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunofluorsescence (IF), Western blot (WB)
Recommended dilution 1: 500 (IF), 1: 2000 -1:6000 (WB)
Expected | apparent MW 32 | 35 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-PYK-10 (C-terminal) antibodies


Arabidopsis thaliana crude leaf from 7 day-old seedlings was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. 10 µg of protein was loaded and separated on 12.5 % SDS-PAGE and blotted to PVDF membrane at 15 V overnight. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.

Additional information

Related products

Background

Background PBP1 (PYK10-binding protein 1) is inhibitor-type lectin that may regulate the correct polymerization of BGLU23/PYK10 upon tissue damage. Activates BGLU21, BGLU22 and BGLU23. PBP1 is localised to cytoplasm.
Alternative names: Jacalin-related lectin 30, Jasmonic acid-induced protein.

Product citations

Selected references Nagano et al. (2005). Activation of an ER-body-localized beta-glucosidase via a cytosolic binding partner in damaged tissues of Arabidopsis thaliana. Plant Cell Physiol. 2005 Jul;46(7):1140-8. doi: 10.1093/pcp/pci126. (Immunofluorescence, Western Blot, Arabidopsis thaliana)
calculated | apparent molecular mass [kDa]: 32 | 35 kDa
Clonality: Polyclonal
Format: Liquid at 2 mg/ml.
Host: Rabbit
immunogen: Conjugated peptide, derived from C-terminus of Arabidopsis thaliana PBP1, UniProt: O04314, TAIR: At3g16420
Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Quantity: 200 ĩg
recommended dilution: 1: 500 (IF), 1: 2000 -1:6000 (WB)
storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Immunofluorsescence (IF), Western blot (WB)
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background: PBP1 (PYK10-binding protein 1) is inhibitor-type lectin that may regulate the correct polymerization of BGLU23/PYK10 upon tissue damage. Activates BGLU21, BGLU22 and BGLU23. PBP1 is localised to cytoplasm.
Alternative names: Jacalin-related lectin 30, Jasmonic acid-induced protein.
Picture (footer): Western blot using anti-PYK-10 (C-terminal) antibodies


Arabidopsis thaliana crude leaf from 7 day-old seedlings was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. 10 µg of protein was loaded and separated on 12.5 % SDS-PAGE and blotted to PVDF membrane at 15 V overnight. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
All references: Nagano et al. (2005). Activation of an ER-body-localized beta-glucosidase via a cytosolic binding partner in damaged tissues of Arabidopsis thaliana. Plant Cell Physiol. 2005 Jul;46(7):1140-8. doi: 10.1093/pcp/pci126. (Immunofluorescence, Western Blot, Arabidopsis thaliana)

Related products: PBP1 | PYK10-binding protein 1 (C-terminal)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €
AS20 4414 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thali...
349 €
AS20 4409 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thali...
323 €