PBP1 | PYK10-binding protein 1 (C-terminal)
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Arabidopsis thaliana crude leaf from 7 day-old seedlings was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. 10 µg of protein was loaded and separated on 12.5 % SDS-PAGE and blotted to PVDF membrane at 15 V overnight. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
Alternative names: Jacalin-related lectin 30, Jasmonic acid-induced protein.
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