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RFP | Red Fluorescent Protein

AS15 3028  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity:  native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.

RFP | Red Fluorescent Protein in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS15 3028)

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product information
Background RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.

Immunogen

KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide

Host Mouse
Clonality Monoclonal
Clone IgG1, clone RF5R
Purity
Format Lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.

Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Related products

other tag antibodies to flourescent proteins

other tag antibodies

Secondary antibodies

Additional information Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.
application information
Recommended dilution 1 : 1000-3000 (WB)
Expected | apparent MW
Confirmed reactivity Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Antibody is purified on Protein A.

Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.
Selected references

to be added when available antibody available in October 2015



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