Anti-RFP | Red Fluorescent Protein (clone RF5R)
Product no: AS15 3028
AS15 3028 | Clonality: monoclonal | Host: Mouse | Reactivity: Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.
282
€
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- Product Info
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Sub class: IgG1 Immunogen: KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide
Host: Mouse Clonality: Monoclonal Purity: Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide. Format: Liquid Quantity: 50 µg Storage: Store at -20°C for up to 2 years. Tested applications: Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 1000-3000 (WB) - Reactivity
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Confirmed reactivity: Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally Additional information (application): Antibody is purified on Protein A.
Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature. - Background
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Background: RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection. - Product Citations
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Selected references: Cecchini et al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031. - Reviews:
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