IKP

RFP | Red Fluorescent Protein (clone RF5R)

Product no: AS15 3028

AS15 3028  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity:  native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.

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  • Product Info
  • Immunogen:

    KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide

    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide.
    Format: Liquid
    Quantity: 50 g
    Storage: Store at -20C for up to 2 years.
    Tested applications: Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 1000-3000 (WB)
  • Reactivity
  • Confirmed reactivity: Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
    Additional information (application): Antibody is purified on Protein A.

    Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.
  • Background
  • Background: RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.

  • Product Citations
  • Selected references: Cecchini et al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031.
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