RFP | Red Fluorescent Protein (clone RF5R)

Name: RFP | Red Fluorescent Protein (clone RF5R)
Brand: IKP
SKU: AS15 3028
Price: 259 EUR
Availability: InStock

AS15 3028 | Clonality: monoclonal | Host: Mouse | Reactivity: native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.

RFP | Red Fluorescent Protein (clone RF5R) in the group Tag Antibodies / GFP/EGFP/BFP/CFP/YFP/RFP at Agrisera AB (Antibodies for research) (AS15 3028)

Product Information

Immunogen

KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide.

Format Liquid

Quantity 50 µg

Storage Store at -20°C for up to 2 years.

Tested applications Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-3000 (WB)

Reactivity

Confirmed reactivity Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally

Antibody is purified on Protein A.

Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.

Background

Background RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.

Product citations

Selected references Cecchini et al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031.

Related products: RFP | Red Fluorescent Protein (clone RF5R)

C-YFP | C-terminal of YFP

AS11 1775 | Clonality: Polyclonal | Host: Rabbit | Reactivity:C-terminal of YFP

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N-YFP | N-terminal of YFP

AS11 1776 | Clonality: Polyclonal | Host: Rabbit | Reactivity:N-terminal of YFP

302 €

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immunogen:	KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide
Sub class:	IgG1
Host:	Mouse
Clonality:	Monoclonal
Purity:	Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide.
Format:	Liquid
Quantity:	50 µg
storage:	Store at -20°C for up to 2 years.
tested applications:	Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1 : 1000-3000 (WB)
Confirmed reactivity:	Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
additional information (application):	Antibody is purified on Protein A. Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.
background:	RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.
All references:	Cecchini et al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031.