RFP | Red Fluorescent Protein (clone RF5R)
AS15 3028 | Clonality: monoclonal | Host: Mouse | Reactivity: native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.

Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide.
Format
Liquid
Quantity
50 ĩg
Storage
Store at -20°C for up to 2 years.
Tested applications
Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-3000 (WB)
Reactivity
Confirmed reactivity
Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
Antibody is purified on Protein A.
Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.
Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.
Background
Background
RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.
Product citations
Selected references
Cecchini et al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031.
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