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RFP | Red Fluorescent Protein (clone RF5R)

AS15 3028  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity:  native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.

RFP | Red Fluorescent Protein (clone RF5R) in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS15 3028)

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Product Information

Immunogen

KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
Format Liquid
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.

Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000-3000 (WB)

Reactivity

Confirmed reactivity Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.
Antibody is purified on Protein A.

Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.

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Background

Background RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.

Product citations

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