SAE1a | SUMO-activating enzyme subunit 1A

Product no: AS19 4284

AS19 4284  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana, Zea mays

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  • Product Info
  • Immunogen: Recombinant, full length SAE1a of Arabidopsis thaliana, UniProt:Q8VY78-1, TAIR: At4g24940, overexpressed in E.coli
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C (short tem, months) or at -80C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW: 36,1 | 36 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Zea mays
    Predicted reactivity: Cajanus cajan, Cicer arietinum, Noccaea caerulescens, Parasponia andersonii, Trema orientale
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Background
  • Background: SAE1a (SUMO-activating enzyme subunit 1A) is the dimeric enzyme acts as an E1 ligase for SUMO1 and SUMO2. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. Functionally redundant with its paralog SAE1B.

    Alternative names: SUMO-activating enzyme subunit 1-1, Ubiquitin-like 1-activating enzyme E1A.
  • Product Citations
  • Selected references: Saracco et al. (2007). Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential. Plant Physiol. 2007 Sep;145(1):119-34.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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