SAE1a | SUMO-activating enzyme subunit 1A

Name: SAE1a | SUMO-activating enzyme subunit 1A
SKU: AS19 4284
Price: 319 EUR
Availability: InStock

Product no: AS19 4284

AS19 4284 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

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Product Info

Immunogen:	Recombinant, full length SAE1a of Arabidopsis thaliana, UniProt:Q8VY78-1, TAIR: At4g24940, overexpressed in E.coli
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 �l
Reconstitution:	For reconstitution add 50 �l, of sterile water
Storage:	Store lyophilized/reconstituted at -20�C (short tem, months) or at -80�C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	36,1 \| 36 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana, Zea mays
Predicted reactivity:	Cajanus cajan, Cicer arietinum, Noccaea caerulescens, Parasponia andersonii, Trema orientale Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Background

Background:

SAE1a (SUMO-activating enzyme subunit 1A) is the dimeric enzyme acts as an E1 ligase for SUMO1 and SUMO2. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. Functionally redundant with its paralog SAE1B.

Alternative names: SUMO-activating enzyme subunit 1-1, Ubiquitin-like 1-activating enzyme E1A.

Product Citations

Selected references:

Saracco et al. (2007). Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential. Plant Physiol. 2007 Sep;145(1):119-34.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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