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SAE1a | SUMO-activating enzyme subunit 1A

AS19 4284  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana, Zea mays

SAE1a | SUMO-activating enzyme subunit 1A in the group Antibodies for Plant/Algal  / Protein Modifications / Autophagy-related and Ubiquitin-like Proteins at Agrisera AB (Antibodies for research) (AS19 4284)

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Product Information

Immunogen Recombinant, full length SAE1a of Arabidopsis thaliana, UniProt:Q8VY78-1, TAIR: At4g24940, overexpressed in E.coli
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 36.1 | 36 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Cajanus cajan, Cicer arietinum, Noccaea caerulescens, Parasponia andersonii, Trema orientale
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Related products

Related products AS08 307 | Anti-UBQ11 | Ubiquitin, rabbit antibodies
AS10 307A | Anti-UBQ11 | Ubiquitin, affinity purified, rabbit antibodies
AS08 308 | Anti-SUMO1 | Small ubiquitin-like modifier protein 1, rabbit antibodies
AS08 349  | Anti-SUMO3 | Small ubiquitin-like modifier protein 3 (peptide antibody), rabbit antibodies
AS19 4283 | Anti-SUMO3 | Small ubiquitin-like modifier protein 3 (protein antibody), rabbit antibodies

Background

Background SAE1a (SUMO-activating enzyme subunit 1A) is the dimeric enzyme acts as an E1 ligase for SUMO1 and SUMO2. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. Functionally redundant with its paralog SAE1B.

Alternative names: SUMO-activating enzyme subunit 1-1, Ubiquitin-like 1-activating enzyme E1A.

Product citations

Selected references Saracco et al. (2007). Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential. Plant Physiol. 2007 Sep;145(1):119-34.

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