Anti-Sumo Tag (monoclonal, clone 1A52F8)

Name: Anti-Sumo Tag (monoclonal, clone 1A52F8)
SKU: AS25 5109
Price: 169 EUR
Availability: InStock

Product no: AS25 5109

AS25 5109 | Clonality: Monoclonal | Host: Mouse | Reactivity: Sumo-tagged proteins

169 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Sub class:	IgG1
Immunogen:	Sumo tag protein
Host:	Mouse
Clonality:	Monoclonal
Purity:	>95%, Protein G purified
Format:	Liquid
Quantity:	50 µg
Storage:	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	ELISA (ELISA), Western Blot (WB)
Recommended dilution:	1:500-1:5000 (WB)
Expected \| apparent MW:	Depends upon MW of fusion partner

Application Examples

SUMO Tagged fusion protein (20 ng/ml) was subjected to SDS-PAGE followed by Western Blot with anti-SUMO tag antibody at a dilution of:
Lane 1:1000
Lane 2:2000
Lane 3:4000
Lane 4:8000
Lane 5:16000
Lane 6:32000
Lane 7:64000
Lane 8:128000
Lane 9:256000
Secondary: Goat polyclonal to Mouse IgG at 1:5000 dilution

Calculated and apparent MW is 55 kDa.

Additional Information
Additional information (application): Antibody is present in 0.01M PBS, pH 7.4, containing 0.03% Proclin 300 as preservative and 50% Glycerol.
Background

Background:

SUMO tag monoclonal antibody is designed to specifically recognize and bind to the SUMO (Small Ubiquitin-like Modifier) tag. The SUMO tag is a peptide sequence derived from the SUMO protein, which is used in recombinant protein expression to facilitate the detection, purification, and characterization of tagged proteins.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Posters Collection
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