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Anti-Sumo Tag Monoclonal Antibody
Product no: AS25 5109
AS25 5109 | Clonality: Monoclonal | Host: Mouse | Reactivity: Sumo-tagged proteins
169
€
Customer reviews
Delivery:
3-6 business days
- Product Info
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Sub class: IgG1 Immunogen: Sumo tag protein sequence Host: Mouse Clonality: Monoclonal Purity: >95%, Protein G purified Format: Liquid Quantity: 50 µg Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.Tested applications: ELISA (ELISA), Western Blot (WB) Recommended dilution: 1:500-1:5000 (WB) Expected | apparent MW: Depends upon MW of fusion partner - Application Examples
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SUMO tagged fusion protein (20 ng/ml) was subjected to SDS-PAGE followed by Western Blot with anti-SUMO tag antibody at a dilution of:
Lane 1:1000
Lane 2:2000
Lane 3:4000
Lane 4:8000
Lane 5:16 000
Lane 6:32 000
Lane 7:64 000
Lane 8:12 8000
Lane 9:25 6000
SUMO tagge fusion protein was separated on Tris-Glycine 12 % SDS-PAGE in reduced conditions at 40 mA followed by a semi-dry transfer at 60 mA for 80 minutes (0.45mm PVDF membrane purchased from Millipore). Blocking was done with 5% skim milk in PBS at RT/1h. Primary antibody was diluted in 0.05% PBST + 2.5% skim milk powder and incubated ON/4°C in a dilution of 1: 5000. Washing was done with 0.1% PBST (4 times, 10 minutes each) followed by a secondary antibody was diluted to 1:50 000 (0.05% with PBST + 2.5% skim milk powder) and incubated at RT/2h followed by four time washes with 0.1% PBST for 10 minutes each and chemiluminescent reaction development and recording of a signal.
Calculated and apparent MW is 55 kDa. - Additional Information
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Additional information (application): Antibody is present in 0.01M PBS, pH 7.4, containing 0.03% Proclin 300 as preservative and 50% Glycerol. - Background
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Background: SUMO tag monoclonal antibody is designed to specifically recognize and bind to the SUMO (Small Ubiquitin-like Modifier) tag. The SUMO tag is a peptide sequence derived from the SUMO protein, which is used in recombinant protein expression to facilitate the detection, purification, and characterization of tagged proteins. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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