V-ATPase holoenzyme

AS17 4158 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Avevena sativa

Product Information

Immunogen V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992

Host

Rabbit

Clonality

Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications

Western blot (WB)

Recommended dilution

1 : 400 - 1: 1000 (WB)

Expected | apparent MW 100, 44 , 42, 36 , 16, 13 kDa

Reactivity

Confirmed reactivity Avena sativa

Predicted reactivity higher plants

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Antibody reacts with V-ATPase subunits VHAa (100 kDa), A, B, 44 , 42, 36 , 16, 13 kDa, and with Calnexin

Background

Background Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1.

Product citations

Selected references Ward et al (1992).Dissociation and Reassembly of the Vacuolar H-ATPase Complex from Oat Roots. Plant Physiol. 99(1):161-9. doi: 10.1104/pp.99.1.161.