V-ATPase holoenzyme

Product no: AS17 4158

AS17 4158 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Avevena sativa

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  • Product Info
  • Immunogen: V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992
    Host:

    Rabbit

    Clonality:

    Polyclonal

    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications:

    Western blot (WB)

    Recommended dilution:

    1 : 400 - 1: 1000 (WB)

    Expected | apparent MW: 100, 44 , 42, 36 , 16, 13 kDa
  • Reactivity
  • Confirmed reactivity: Avena sativa
    Predicted reactivity: higher plants
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information (application): Antibody reacts with V-ATPase subunits VHAa (100 kDa), A, B, 44 , 42, 36 , 16, 13 kDa, and with Calnexin
  • Background
  • Background: Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1.
  • Product Citations
  • Selected references: Ward et al (1992).Dissociation and Reassembly of the Vacuolar H-ATPase Complex from Oat Roots. Plant Physiol. 99(1):161-9. doi: 10.1104/pp.99.1.161.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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