Anti-APX | L-ascorbate peroxidase

329 €

Product no: AS08 368

MIO
We use Arabidopsis thaliana whole leaf as sample. We did Western blot 1:2000 works wonderful!	2015-02-20 @ 18:07:09

Elzbieta Romanowska
We used this antibody for APX immunodetection analysis (ECL analysis) in the leaves of different subtypes of C4 plants. SDS-PAGE was performed on 15% polyacrylamide gel and 7 ug protein was loaded to each lane. We used 1:2000 dilution. The highest signal was observed in Panicum milliaceum (NAD-ME subtype), Zea mays (NADP-ME subtype) and Echinochloa cruss- galli (NADP-ME). The weakest signal was noted in Digitaria sanguinalis (NADP-ME), but this may be caused by different protein amount in the given conditions.	2013-10-31 @ 08:08:32

Anna
I used this antybody on leaf and mitochondrial extracts and it perfectly worked for both.details: Arabidopsis thaliana, 20ug leaf extract, 10ug mitochondrial extract per lane, 1:2000 dilution, ECL detection.	2013-10-14 @ 14:48:40

Luis E Hernandez
We tried with success this antibody in Medicago sativa, with 10 ug protein loading, with a 1/2000 dilution of primary antibody and 1/20000 secondary HRP, suitable for ECL detection	2011-10-04 @ 21:57:24