Anti-H+ATPase | Plasma membrane H+ATPase (rabbit antibody)

Marina Gavilanes-Ruíz
The H+ATPase (AS07260) antibody was used to evaluated plasma membrane enrichment from Arabidopsis leaves. We used 1:10000 dilution in TTBS buffer supplemented with 2% (w/v) non-fat powdered milk followed for overnight at room temperature with agitation. Detection was developed with anti-rabbit IgG secondary antibody HRP conjugated (1:20000 dilution).	2021-03-25 @ 02:05:18
Amelie Spazierer
We used the H+ATPase antibody for Western experiments at high dilutions (1:5000) as well as for epifluorescence microscopy at lower dilutions (1:250 and 1:50) and always got a pretty good signal. There is some background for both but since the ATPase is highly abundant it is easy to get rid of that.	2020-07-15 @ 11:33:51
Peeyush Ranjan
The H ATPase antibody (AS07 260) was tested on total cell lysate of Chlamydomonas reinhardtii. An increasing concentration from 5ug to 50ug of total protein was loaded in 4-20% gradient gel and transferred on PVDF. After blocking with TBST 2?t free milk, membrane was incubated with 1:5000 dilution of AS07 260 Ab in TBST buffer followed by 1:10000 dilution of secondary Ab after washing. A very specific band was observed even with lowest concentration of protein. For further blotting, I used 1:7500 dilution. Very good specificity with minimum background.	2017-03-07 @ 16:38:50
Anna Bilska
Application: immunolocalization at the electron microscope level (immunogold), dilution: 1:100 (PBS BSA) confirmed reactivity in maize (Zea mays).	2013-07-25 @ 16:40:30
Nicole Clay
Antibody worked great for plasma membrane enrichment. We used 1:1000 dilution on Arabidopsis thaliana extracts that have gone through aqueous 2-phase partitioning	2012-12-17 @ 13:23:27
Dudley Page
A strong and specific signal when used at 1/1000 against purified plasma membranes from Chlamydomonas reinhardtii.	2010-05-05 @ 21:33:21
This antibody was used to detect the H ATPase in the fern Pteris vittata. In order to get decent detection of this protein, the antibody was optimized for a dilution of 1:1000. The antibody was incubated at room temperature (22 deg) for about 8 hours before washing and secondary incubation.	2009-07-21 @ 15:25:18