Goat anti-Rabbit IgG (H&L), HRP conjugated

Shelby Hotz
For my lab's study, we used this antibody for western blots to detect the abundance of Hsp70 in heat shocked and non-heat shocked bull kelp (Nereocystis leutkeana). We used a 1:5000 for the initial dilution and then a 1:2500 dilution after adding glycerol. Both were done in a 5% Nonfat Dried Milk in 1x Tris Buffered Saline solution. The results were positive, with the protein bands showing up clearly and at the correct weight according to the positive control we used.I am happy to report the antibody has been working effectively and as intended in our molecular research. The instructions that come with the product are very comprehensive and relatively easy to follow, and doing so has allowed us to effectively use the materials as expected and needed. We also appreciate the promptness of the delivery and arrival of the materials, and having the antibody in a lyophilized form is very much appreciated.Thank you for your service.	2023-03-21 @ 19:02:34
Vasundara Sundar
We are very satisfied with the goat anti-rabbit antibodies, it worked well with all of the primary antibodies we used. 1:10000 dilution worked well for us.	2022-12-28 @ 11:40:31
Annika Brünje
This antibody gives very good results in my Western blot analyses and can be also used in combination with primary antibodies that are a bit more tricky in their application. Usually, I use it in a dilution of 1:10000 to detect proteins in Arabidopsis leaf extracts.	2021-01-19 @ 14:20:04
Soo Yeon Ko
I was satisfied with goat anti-rabbit IgG antibodies on protein extracts from Oryza Sativa in dilution : 1: 25 000	2020-05-28 @ 15:37:49
?udmila Holubová
We are happy with this antibody, works well with all primary antibodies we used. Our dilution was 1:12500.	2019-10-04 @ 13:38:32
Ella Nukarinen
We are very happy with goat anti-rabbit IgG HRP antibody. We use it for Western blots in dilution 1:10 000-1:20 000 for detecting endogenous proteins from Arabidopsis protein extracts.	2018-05-23 @ 13:03:10
Beata Kmiec
We're very happy with the antibodies. Our regular application is in western blots with Arabidopsis thaliana total protein extract and with isolated mitochondria and chloroplasts, but we've used it with success in WB with yeast and human samples. We use it in dilution 1:20000.	2017-02-13 @ 09:50:45
Malgorzata Kwasniak-Owczarek
We are very satisfied with goat anti-rabbit IgG antibodies on protein extracts from Arabidopsis thaliana in dilution: 1: 25 000.	2016-11-02 @ 21:12:01
Mirka Herbstova
I used Goat-anti rabbit IgG HRP conjugated secondary antibody (AS 09 602) at a dilution 1:50 000 for alga species Phaeodactylum and Trachydiscus without cross-reactions.	2016-04-22 @ 12:02:21
Jakub Dolata, Adam Mickiewicz University, Poznan, Poland
The goat anti-rabbit IgG-HRP from Agrisera are very nice working antibodies with no unspecific signals in different dillutions (1:50000, 1:100000). We are using them in case of plant (Arabidopsis, Solanum) and human cells extracts as well.	2014-03-12 @ 13:30:19
Jakub DOlata
The goat anti-rabbit IgG-HRP from Agrisera are very nice working antibodies with no unspecific signals in different dillutions (1:50000	2014-03-12 @ 13:25:40
Gerhard Obermeyer, Univ Salzburg, Austria
We were looking for a 2nd anti-rabbit antibody which does not cross-react with proteins from a crude lily pollen lysate. The goat anti-rabbit IgG-HRP from Agrisera was the only one which showed no unspecific signals and could be used in a 1:40,000 dilution. All other antibodies from 5 different sources cross-reacted with a high number of proteins in the pollen lysate.	2011-11-11 @ 09:15:07