Anti-RbcL | Rubisco large subunit, form I (rabbit)

Orly Levitan
I have been using this antibody for the past 7 years. It has worked wonderfully for me every time I tried. I have used it on cyanobacteria (including Trichodesmium), Diatoms, and Seagrasses, with and without the RbcL standard. i have several publication in which I used this antibody (and others by Agrisera). Publication for example: Levitan et al., 2010, Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations, Environmental microbiology.Levitan et al., 2015, Remodeling of intermediate metabolism in the diatom Phaeodactylum tricornutum under nitrogen stress, PNAS	2016-09-14 @ 04:06:02
Jerzy Kruk
samples used were thylakoids and leaf powder, 12% SDS gels for PAGE, 5 ug protein per lane or 7 ug/chlorophyll per lane (thylakoids), dilution 1:5000, the observed product at ca. 53 kDa, AB works well	2014-02-10 @ 17:46:07
Renata Ditt
Weve been very happy with this antibody. We have been using it for both westerns and ELISAs, with great results. We work with sea water samples containing a mixed population of species of diatoms and other plankton.	2013-11-26 @ 00:52:15
Katarzyna Hura
I am satisfied with this antibody from Agrisera. It gives clean results. We have used this antibody for western blot detection (diluttion 1:20 000).	2013-10-30 @ 13:23:04
Tanja Göbel
I´m using the antibodies for the immunodetection on western blots with the fluorescent based licor odyssey system (with the IRDye secondary antibodies Donkey anti rabbit IgG 800CW and 680 (Licor)). I´m analysing whole protein or chloroplasts protein extracts optained from Arabidopsis thaliana. Proteins were either precipitated with TCA/Aceton or directly solubilized with NP-40 in a tris-buffer. The only problem with the unprecipitated samples is that one gets a very strong backround in the 680nm channel due to the chlorophyll-fluorescence. The primary antibodies were diluted in TBS with 0,02% Sodiumazid according to the recommanded dilution (for ECL) in the data sheet and the membranes were incubated for 1 h at RT with agitation. The membranes are blocked with BSA in TBS and washed with TBS-T. I´m reusing the diluted antibodies for up to 5 to 10 western blots without any significant loss of signal (diuluted antibodies are stored at 4°C). The signals I´m getting with the antibodies are very clear and hieghly specific, with only low (anti-Lhcb1 and 2) or nearly no (anti-RbcL and RA) unspecific binding. Usually, I´m loading 10 µg protein and seperate them on 10% Mini-gels. So far I had no problems with Agrisera-antibodies.	2012-09-25 @ 13:16:14
Sarah Croonenborghs
The antibody worked excellent on a Guzmania hybrid (Bromeliaceae) in both dilutions, 1: 5000 and 1:10 000.	2009-05-28 @ 10:34:54
Enrique Olmos Aranda. CEBAS-CSIC. Spain
We have satisfactorily used this antibody with Paspalum and tobacco for immunolabelling.Dilution: 1/250	2009-02-20 @ 12:23:34