Anti-PsaB | PSI-B core subunit of photosystem I

Monika Ostaszewska
Good detection on A.thaliana thylakoid membrane fraction samples containing 1 ug of chlorophyll per well on 10% gel (primary antibody concentration 1:1000).	2021-08-16 @ 13:27:39
Katya Georgieva
Worked very well on thylakoid proteins from Haberlea rhodopensis. We used 3 ug chlorophyll per lane, 1:1000 dilution of PsaB, 1:25000 dilution of 2nd antibody, ECL detection of WB. Reference: Mihailova G, Büchel C, Dietzel L, Georgieva K (2016) Desiccation induced changes in photosynthesis related proteins of shade and sun Haberlea rhodopensis plants. Compt. Rend Bulg. Acad. Sci. 69(1), 37-44	2019-06-24 @ 09:09:12
Dimitris Petroutsos
We obtained very good results using whole cell exctracts of Phaeodactylum tricornutum. Here are the details: 4 µg of total protein from Phaeodactylum tricornutum were loaded on a 13% SDS-PAGE and blotted 1.5 h to nitrocellulose. Blots were blocked immediately following transfer in 5% milk-TBS-T for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1:2000 overnight at 4 oC with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBS-T for 1h at room temperature with agitation. The blots were washed as above and developed 6 sec with ECL detection reagent. Images of the blots were obtained using a CCD imager (Chemidock MP Imaging, Bio-Rad).	2014-12-20 @ 23:03:18
Maya Velitchkova
We obtain very good results using this antibody with thylakoid membranes from peas and tomato and PSI particles from spinach.	2014-04-30 @ 13:36:06
Amy Verhoeven
Works fairly well for pumpkin at 1:500 with 10 ug protien loaded but this dilution is very high. Similar results with Quercus alba and Acer rubrum thylakoids.	2011-01-14 @ 18:46:47