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LEA4-5 (78-158) | Late embryogenesis abundant protein 4-5 (C-terminal)

AS22 4831 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

LEA4-5 (78-158) | Late embryogenesis abundant protein 4-5 (C-terminal) in the group Antibodies Plant/Algal  / Environmental Stress / Drought stress at Agrisera AB (Antibodies for research) (AS22 4831)



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Product Information

Immunogen Part of a recombinant Arabidopsis thaliana LEA4-5, corresponding to position 78-158, UniProt: Q9FG31 , TAIR: AT5G06760
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

16 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot with anti-LEA4-5 (78-158) antibodies

The amount of purified protein or peptide used in these dot blot experiments is indicated in the scheme on the right side of the image (2 ng to 200 ng). As specificity controls, we used 200 ng of purified AtLEA4-5 and of a peptide corresponding to the AtLEA4-5 C-region. In all cases equal volumes of the different samples were dotted on Nitrocellulose Blotting Membrane 0.45 µm Amersham TM Protran TM Premium. Once samples were dry, membranes were blocked with non-fat milk 5% (W/V) during eight hours at 4°C with agitation. Blots were incubated with primary antibody at the indicated dilution for two days at 4°C with slow agitation. The antibody solution was decanted, and blots were rinsed briefly, then washed once for 15 min and 2-times for 5 min in TBS-T at room temperature (RT) with agitation. Subsequently, blots were incubated with secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 for 8 h at RT with agitation. The blot was washed as described above and developed for 2 min with using chemiluminescent detection reagent according to manufacture recommendations.. Exposure time was 60 seconds.

Courtesy of Dr. Alejandra A. Covarrubias,Universidad Nacional Autónoma de México, Mexico

Additional information

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Background

Background

LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.

Product citations

background:

LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.

Picture (footer): Western blot with anti-LEA4-5 (78-158) antibodies

The amount of purified protein or peptide used in these dot blot experiments is indicated in the scheme on the right side of the image (2 ng to 200 ng). As specificity controls, we used 200 ng of purified AtLEA4-5 and of a peptide corresponding to the AtLEA4-5 C-region. In all cases equal volumes of the different samples were dotted on Nitrocellulose Blotting Membrane 0.45 µm Amersham TM Protran TM Premium. Once samples were dry, membranes were blocked with non-fat milk 5% (W/V) during eight hours at 4°C with agitation. Blots were incubated with primary antibody at the indicated dilution for two days at 4°C with slow agitation. The antibody solution was decanted, and blots were rinsed briefly, then washed once for 15 min and 2-times for 5 min in TBS-T at room temperature (RT) with agitation. Subsequently, blots were incubated with secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 for 8 h at RT with agitation. The blot was washed as described above and developed for 2 min with using chemiluminescent detection reagent according to manufacture recommendations.. Exposure time was 60 seconds.

Courtesy of Dr. Alejandra A. Covarrubias,Universidad Nacional Autónoma de México, Mexico

calculated | apparent molecular mass [kDa]:

16 kDa

Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen: Part of a recombinant Arabidopsis thaliana LEA4-5, corresponding to position 78-158, UniProt: Q9FG31 , TAIR: AT5G06760
Purity: Serum
Quantity: 50 µl
recommended dilution: 1 : 2000 (WB)
Reconstitution: For reconstitution add 50 µl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known

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