LEA4-5 (78-158) | Late embryogenesis abundant protein 4-5 (C-terminal)
AS22 4831 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
Part of a recombinant Arabidopsis thaliana LEA4-5, corresponding to position 78-158, UniProt: Q9FG31 , TAIR: AT5G06760
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
16 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
The amount of purified protein or peptide used in these dot blot experiments is indicated in the scheme on the right side of the image (2 ng to 200 ng). As specificity controls, we used 200 ng of purified AtLEA4-5 and of a peptide corresponding to the AtLEA4-5 C-region. In all cases equal volumes of the different samples were dotted on Nitrocellulose Blotting Membrane 0.45 µm Amersham TM Protran TM Premium. Once samples were dry, membranes were blocked with non-fat milk 5% (W/V) during eight hours at 4°C with agitation. Blots were incubated with primary antibody at the indicated dilution for two days at 4°C with slow agitation. The antibody solution was decanted, and blots were rinsed briefly, then washed once for 15 min and 2-times for 5 min in TBS-T at room temperature (RT) with agitation. Subsequently, blots were incubated with secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 for 8 h at RT with agitation. The blot was washed as described above and developed for 2 min with using chemiluminescent detection reagent according to manufacture recommendations.. Exposure time was 60 seconds.
The amount of purified protein or peptide used in these dot blot experiments is indicated in the scheme on the right side of the image (2 ng to 200 ng). As specificity controls, we used 200 ng of purified AtLEA4-5 and of a peptide corresponding to the AtLEA4-5 C-region. In all cases equal volumes of the different samples were dotted on Nitrocellulose Blotting Membrane 0.45 µm Amersham TM Protran TM Premium. Once samples were dry, membranes were blocked with non-fat milk 5% (W/V) during eight hours at 4°C with agitation. Blots were incubated with primary antibody at the indicated dilution for two days at 4°C with slow agitation. The antibody solution was decanted, and blots were rinsed briefly, then washed once for 15 min and 2-times for 5 min in TBS-T at room temperature (RT) with agitation. Subsequently, blots were incubated with secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 for 8 h at RT with agitation. The blot was washed as described above and developed for 2 min with using chemiluminescent detection reagent according to manufacture recommendations.. Exposure time was 60 seconds.
Courtesy of Dr. Alejandra A. Covarrubias,Universidad Nacional Autónoma de México, Mexico
Additional information
Background
Background
LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.
Product citations
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