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PSA2 | Photosystem I assembly factor 2

AS13 2654 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PSA2 | Photosystem I assembly factor 2 in the group Antibodies for Plant/Algal  / Photosynthesis  / GreenCut at Agrisera AB (Antibodies for research) (AS13 2654)

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Data sheet Product citations Protocols Add review

Product Information

Immunogen recombinant protein corresponding to amino acids 87 to 186 of Arabidopsis thaliana PSA2, UniProt:O64750, TAIR: AT2G34860
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

20 kDa | 10 kDa (processed form)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica rapa
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-PSA2 antibodies

Respective amounts of a leaf total cell extract from Arabidopsis thaliana loaded on total chlorophyll µg/µl were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 10 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.

Courtesty of Dr. Rikard Fristedt, Biophysics of Photosynthesis, Dep. Physics and Astronomy, Faculty of Sciences. VU University of Amsterdam, The Netherlands

Additional information

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Background

Background PSA2 (Photosystem I assembly factor 2) is a protein coded by a gene belonging to the "Green Cut" set, found only in green algae and plants but not in non-photosynthetic organisms. PSA2 is localized in thylakoid lumen.

Product citations

Selected references Fristedt et al. (2014). A Thylakoid Membrane Protein Harboring a DnaJ-type Zinc Finger Domain is Required for Photosystem I Accumulation in Plants. J Biol Chem. 2014 Sep 16. pii: jbc.M114.587758.

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