2b protein [Tomato aspermy virus]
AS16 3982 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Tomato aspermy virus
![2b protein [Tomato aspermy virus] in the group Antibodies Plant/Algal / Plant Pathogens / Viruses at Agrisera AB (Antibodies for research) (AS16 3982)](/bilder/artiklar/zoom/AS16 3982_1.jpg?m=1636107013)
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Product Information
Immunogen
Recombinant 2b protein [Tomato aspermy virus] Protein accession number: NP_620826.
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western Blot (WB)
Recommended dilution
1 : 5000 (WB)
Expected | apparent MW
11,2 | 17 kDa
Reactivity
Confirmed reactivity
2b protein [Tomato aspermy virus]
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example
![western blot using anti-2b protein [Tomato aspermy virus] antibody](https://www.agrisera.com/dokument/bibliotek/Image/AS16_3982.jpg)
The indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions. Exposure time was 20 seconds.
Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA
The indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions. Exposure time was 20 seconds.
Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA
Additional information
Background
Product citations
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