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2b protein [Tomato aspermy virus]

AS16 3982 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Tomato aspermy virus

2b protein [Tomato aspermy virus] in the group Antibodies Plant/Algal  / Plant Pathogens / Viruses at Agrisera AB (Antibodies for research) (AS16 3982)
2b protein [Tomato aspermy virus]



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Product Information

Immunogen Recombinant 2b protein [Tomato aspermy virus] Protein accession number: NP_620826.
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western Blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW 11,2 | 17 kDa

Reactivity

Confirmed reactivity 2b protein [Tomato aspermy virus]
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-2b protein [Tomato aspermy virus] antibody
The indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions. Exposure time was 20 seconds.

Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA

Additional information

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