Name:
Phone:
E-mail:
Address:


 


2b protein [Tomato aspermy virus]

AS16 3982 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Tomato aspermy virus

2b protein [Tomato aspermy virus] in the group Antibodies for Plant/Algal  / Plant Pathogens / Viruses at Agrisera AB (Antibodies for research) (AS16 3982)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant 2b protein [Tomato aspermy virus] Protein accession number: NP_620826.
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western Blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

11.2 | 17 kDa

Reactivity

Confirmed reactivity 2b protein [Tomato aspermy virus].
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-2b protein [Tomato aspermy virus] antibody
The indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions. Exposure time was 20 seconds.

Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA

Additional information

Related products

Background

Product citations

Related products: 2b protein [Tomato aspermy virus]

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS16 ECL-S | extreme low femtogram detection
- 10ml trial size is restricted to one unit per cust...
From 10 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
195 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €