2S3M | 2S seed storage protein 3 (2S3 Albumin)

AS20 4404 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

2S3M | 2S seed storage protein 3 (2S3 Albumin)

Product Information

Immunogen Conjugated peptide, derived of Arabidopsis thaliana 2S3 large subunit, UniProt: P15459, TAIR: At4g27160

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format Liquid at 2 mg/ml.

Quantity 200 µg

Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunolocalisation by electron microscopy (IL), Western blot (WB)

Recommended dilution assay dependent (ELISA), 1: 500 (IL), 1: 10 000 - 1: 20 000 (WB)

Expected | apparent MW 18, 7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Capsella rubella
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-2S3M antibodies

Arabidopsis thaliana dry seed extract from wilde-type (1) and mutants that accumulate precursors of major storage proteins: mag5-1, mag2-1 and mag4-1 (2-4) were separated on 15-20 % SDS-PAGE and blotted to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2500 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
p2S - precursor of 2S; 2S-L - large subunit of 2S

Additional information

Background

Background 2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds. Subcellular localisation: vacuole.

Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3

Product citations

Selected references Takagi et al. (2013). MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell . 2013 Nov;25(11):4658-75. doi: 10.1105/tpc.113.118158. (Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)

Picture (footer):	Arabidopsis thaliana dry seed extract from wilde-type (1) and mutants that accumulate precursors of major storage proteins: mag5-1, mag2-1 and mag4-1 (2-4) were separated on 15-20 % SDS-PAGE and blotted to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2500 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations. p2S - precursor of 2S; 2S-L - large subunit of 2S
calculated \| apparent molecular mass [kDa]:	18, 7 kDa
Clonality:	Polyclonal
Format:	Liquid at 2 mg/ml.
Host:	Rabbit
immunogen:	Conjugated peptide, derived of Arabidopsis thaliana 2S3 large subunit, UniProt: P15459, TAIR: At4g27160
Purity:	Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Quantity:	200 µg
recommended dilution:	assay dependent (ELISA), 1: 500 (IL), 1: 10 000 - 1: 20 000 (WB)
storage:	Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunolocalisation by electron microscopy (IL), Western blot (WB)
All references:	Takagi et al. (2013). MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell . 2013 Nov;25(11):4658-75. doi: 10.1105/tpc.113.118158. (Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)
background:	2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds. Subcellular localisation: vacuole. Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Capsella rubella Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known

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