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2S3M | 2S seed storage protein 3 (2S3 Albumin)

AS20 4404 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana
2S3M | 2S seed storage protein 3 (2S3 Albumin) in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS20 4404)

DATA SHEET IN PDF

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330
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Data sheet Product citations Protocols Add review

Product Information

Immunogen Conjugated peptide, derived of Arabidopsis thaliana 2S3 large subunit, UniProt: P15459, TAIR: At4g27160
Host Rabbit
Clonality Polyclonal
Purity Total IgG, purified on Protein A
Format Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Quantity 200 µg
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunolocalisation by electron microscopy (IL), Western blot (WB)
Recommended dilution assay dependent (ELISA), 1: 500 (IL), 1: 10 000 - 1: 20 000 (WB)
Expected | apparent MW 18. 7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Capsella rubella
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using anti-2S3M antibodies

Arabidopsis thaliana dry seed extract from wilde-type (1) and mutants that accumulate precursors of major storage proteins: mag5-1, mag2-1 and mag4-1 (2-4) were separated on 15-20 % SDS-PAGE and blotted to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2500 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
p2S - precursor of 2S; 2S-L - large subunit of 2S

Additional information

Related products

Background

Background 2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds. Subcellular localisation: vacuole.

Alternative names:  2S albumin storage protein, NWMU2-2S albumin 3

Product citations

Selected references Takagi et al. (2013). MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell . 2013 Nov;25(11):4658-75. doi: 10.1105/tpc.113.118158. (Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)

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