2S3P | 2S seed storage protein 3
AS20 4405 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Conjugated peptide, derived from N-propeptide of Arabidopsis thaliana 2S3P, UniProt: P15459, TAIR: At4g27160
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format
Liquid at 2 mg/ml.
Quantity
200 ĩg
Storage
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunolocalisation by electron microscopy (IL), Western blot (WB)
Recommended dilution
assay dependent (ELISA), 1: 500 (IL), 1: 2500 (WB)
Expected | apparent MW
18,7 | 17-20 kDa (2S albumin precursors)
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples

Arabidopsis thaliana dry seeds from wilde-type (WT) and mag2-2 mutant (defective in processing of the precursors) were homogenised in SDS sample buffer (100 mM Tris/HCl, pH 6.8, 4 % SDS w/v, 20 % glycerol v/v, 10 % 2-mercaptoethanol) and separated on SDS-PAGE and blotted to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
Anti-2S3P antibodies are recognizing 2S albumin precursors (p2S) but not the mature forms (2S).

Arabidopsis thaliana dry seeds from wilde-type (WT) and mag2-2 mutant (defective in processing of the precursors) were homogenised in SDS sample buffer (100 mM Tris/HCl, pH 6.8, 4 % SDS w/v, 20 % glycerol v/v, 10 % 2-mercaptoethanol) and separated on SDS-PAGE and blotted to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
Anti-2S3P antibodies are recognizing 2S albumin precursors (p2S) but not the mature forms (2S).
Additional information
Anti-2S3P antibodies are recognizing 2S albumin precursors (p2S) but not the mature forms (2S)
Background
Background
2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds. Subcellular localisation: vacuole.
Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3
Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3
Product citations
Selected references
Shirakawa et al. (2014). CONTINUOUS VASCULAR RING (COV1) is a trans-Golgi network-localized membrane protein required for Golgi morphology and vacuolar protein sorting. Plant Cell Physiol. 2014 Apr;55(4):764-72. doi: 10.1093/pcp/pct195. (Western blot, Arabidopsis thaliana)
Li et al. (2006). MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana. Plant Cell. 2006 Dec;18(12):3535-47. doi: 10.1105/tpc.106.046151.( Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)
Li et al. (2006). MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana. Plant Cell. 2006 Dec;18(12):3535-47. doi: 10.1105/tpc.106.046151.( Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)
Related products: 2S3P | 2S seed storage protein 3
AS20 4403 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thali...
349 €
AS20 4404 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thali...
349 €
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 26 €
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 ...
10 ...
201 €