Name:
Phone:
E-mail:
Address:


 


2S3P | 2S seed storage protein 3

AS20 4405 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana
2S3P | 2S seed storage protein 3 in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS20 4405)

DATA SHEET IN PDF

Qty: 
330
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Conjugated peptide, derived from N-propeptide of Arabidopsis thaliana 2S3P, UniProt: P15459, TAIR: At4g27160
Host Rabbit
Clonality Polyclonal
Purity Total IgG, purified on Protein A
Format Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Quantity 200 µg
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunolocalisation by electron microscopy (IL), Western blot (WB)
Recommended dilution assay dependent (ELISA), 1: 500 (IL), 1: 2500 (WB)
Expected | apparent MW 18.7 | 17-20 kDa (2S albumin precursors)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using anti-2S3P antibodies

Arabidopsis thaliana dry seeds from wilde-type (WT) and mag2-2 mutant (defective in processing of the precursors) were homogenised in SDS sample buffer (100 mM Tris/HCl, pH 6.8, 4 % SDS w/v, 20 % glycerol v/v, 10 % 2-mercaptoethanol) and separated on SDS-PAGE and blotted to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.

Anti-2S3P antibodies are recognizing 2S albumin precursors (p2S) but not the mature forms (2S).

Additional information

Anti-2S3P antibodies are recognizing 2S albumin precursors (p2S) but not the mature forms (2S).

Related products

Background

Background 2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds. Subcellular localisation: vacuole.

Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3

Product citations

Selected references Shirakawa et al. (2014). CONTINUOUS VASCULAR RING (COV1) is a trans-Golgi network-localized membrane protein required for Golgi morphology and vacuolar protein sorting. Plant Cell Physiol. 2014 Apr;55(4):764-72. doi: 10.1093/pcp/pct195. (Western blot, Arabidopsis thaliana)
Li et al. (2006). MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana. Plant Cell. 2006 Dec;18(12):3535-47. doi: 10.1105/tpc.106.046151.( Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)

Related products: 2S3P | 2S seed storage protein 3

AS20 4403 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thali...
330 €
AS20 4404 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thali...
330 €
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €