IKP

5-fC | 5-formylcytosine

Product no: AS16 3165
AS16 3165 Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:Human, Mouse, other species
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  • Product Info
  • Immunogen: KLH-conjugated 5-fC (5-formylcytosine)
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Liquid
    Quantity: 100 l
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: DIP (DNA Immunoprecipitation (IP), ELISA (ELISA)
    Recommended dilution: 3 µl (DIP), 1 : 10 000 (ELISA)
  • Reactivity
  • Confirmed reactivity: Human
    Predicted reactivity: Mouse, broad species range
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    DIP using polyclonal anti-5-fC | 5-formylcytosine antibodies

    DIP: HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the polyclonal antibody against 5-fC in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).

    ELISA using anti-5-fC | 5-formylcytosine polyclonal antibodies


    ELISA: to determine the titer of the antibody, an ELISA was performed using a serial dilution of the polyclonal antibody directed against 5-fC. The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:100 000.
  • Additional Information
  • Additional information: This serum contains 0,05 % sodium azide
  • Background
  • Background: 5-fC (5-formylcytosine) is a known modification of DNA for epigenetic regulation.
  • Product Citations
  • Selected references: 38221900
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