5-fC | 5-formylcytosine
AS16 3165 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Human, Mouse, other species

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Product Information
Immunogen
KLH-conjugated 5-fC (5-formylcytosine)
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Liquid
Quantity
100 ĩl
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
DIP (DNA Immunoprecipitation (IP), ELISA (ELISA)
Recommended dilution
3 µl (DIP), 1 : 10 000 (ELISA)
Reactivity
Confirmed reactivity
Human
Predicted reactivity
Mouse, broad species range
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example
DIP: HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the polyclonal antibody against 5-fC in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).

ELISA: to determine the titer of the antibody, an ELISA was performed using a serial dilution of the polyclonal antibody directed against 5-fC. The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:100 000.

DIP: HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the polyclonal antibody against 5-fC in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).

ELISA: to determine the titer of the antibody, an ELISA was performed using a serial dilution of the polyclonal antibody directed against 5-fC. The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:100 000.
Additional information
Additional information
This serum contains 0,05 % sodium azide
Background
Background
5-fC (5-formylcytosine) is a known modification of DNA for epigenetic regulation.
Product citations
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