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5-fC | 5-formylcytosine

AS16 3165 Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:Human, Mouse, other species
5-fC | 5-formylcytosine in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3165)

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Product Information

Immunogen KLH-conjugated 5-fC (5-formylcytosine)
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid
Quantity 100 µl
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications DIP (DNA Immunoprecipitation (IP), ELISA (ELISA)
Recommended dilution 3 µl (DIP), 1 : 10 000 (ELISA)

Reactivity

Confirmed reactivity Human
Predicted reactivity Mouse, broad species range
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

DIP using polyclonal anti-5-fC | 5-formylcytosine antibodies

DIP: HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the polyclonal antibody against 5-fC in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).

ELISA using anti-5-fC | 5-formylcytosine  polyclonal antibodies


ELISA: to determine the titer of the antibody, an ELISA was performed using a serial dilution of the polyclonal antibody directed against 5-fC. The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:100 000.

Additional information

Additional information This serum contains 0.05 % sodium azide.

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Background

Background 5-fC (5-formylcytosine) is a known modification of DNA for epigenetic regulation.

Product citations

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