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His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)

AS15 2935 | Clonality: monoclonal | Host: mouse | Reactivity: Polyhistidine tagged proteins (N- or C-terminus label)
His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10) in the group Tag Antibodies / His (3x, 6x) at Agrisera AB (Antibodies for research) (AS15 2935)



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Product Information

Immunogen

Polyhistidine (HHHHHH) tagged polypeptide

Host Mouse
Clonality Monoclonal
Purity Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.
Format Lyophilized
Quantity 100 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Tested applications Western blot (WB)
Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Application examples

Additional information

Additional information

Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, 2 mM Sodium Azide (pH 7.4)

Concentration: 0.1mg/ml

Related products

Background

Background Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.

Product citations

immunogen:

Polyhistidine (HHHHHH) tagged polypeptide

Reconstitution: For reconstitution add 100 ĩl of sterile water
Host: Mouse
Clonality: Monoclonal
Purity: Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.
Format: Lyophilized
Quantity: 100 ĩg
storage: Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
tested applications: Western blot (WB)
recommended dilution: The optimal working dilution should be determined by the investigator
additional information:

Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, 2 mM Sodium Azide (pH 7.4)

Concentration: 0.1mg/ml

background: Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.

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