His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)

Product no: AS15 2935
AS15 2935 | Clonality: monoclonal | Host: mouse | Reactivity: Polyhistidine tagged proteins (N- or C-terminus label)
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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Polyhistidine (HHHHHH) tagged polypeptide

    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.
    Format: Lyophilized
    Quantity: 100 µg
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
    Tested applications: Western blot (WB)
    Recommended dilution: 1: 5000 - 10 000 (WB)
    Expected | apparent MW: depends upon fusion partner
  • Reactivity
  • Confirmed reactivity: 6xHis tagged proteins
  • Application Examples
  • Western blot using anti-6xHis monoclonal antibodies

    Indicated amount of 6xHis tagged, 60 kDa protein has been loaded to each lane. Primary antibody incubation (AS15 2935): 1: 10 000 1h/RT Followed by three washes and reaction was developed using alkaline phosphatase substrate.

  • Additional Information
  • Additional information:

    Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, 2 mM Sodium Azide (pH 7.4)

    Concentration: 0.1mg/ml

    Additional information (application): Final primary antibody dilution, depend upon amount of Hisx6 tagged protein in analyzed sample. 
  • Background
  • Background: Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
  • Product Citations
  • Selected references: 38221900
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