His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)

Product no: AS15 2935
AS15 2935 | Clonality: monoclonal | Host: mouse | Reactivity: Polyhistidine tagged proteins (N- or C-terminus label)
455
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Polyhistidine (HHHHHH) tagged polypeptide

    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.
    Format: Lyophilized
    Quantity: 100 µg
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
    Tested applications: Western blot (WB)
    Recommended dilution: 1: 5000 - 10 000 (WB)
    Expected | apparent MW: depends upon fusion partner
  • Reactivity
  • Confirmed reactivity: 6xHis tagged proteins
  • Application Examples
  • Western blot using anti-6xHis monoclonal antibodies

    Indicated amount of 6xHis tagged, 60 kDa protein has been loaded to each lane. Primary antibody incubation (AS15 2935): 1: 10 000 1h/RT Followed by three washes and reaction was developed using alkaline phosphatase substrate.

  • Additional Information
  • Additional information:

    Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, 2 mM Sodium Azide (pH 7.4)

    Concentration: 0.1mg/ml

    Additional information (application): Final primary antibody dilution, depend upon amount of Hisx6 tagged protein in analyzed sample. 
  • Background
  • Background: Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

img missing

AS11 1771   | Clonality: monoclonal  |  Host: Mouse  |  Reactivity:His-tag

274 €
Add to cart
Info
Buy 2 items of this product for 187.00 €/items
Buy 3 items of this product for 171.00 €/items
img missing

AS19 4255   | Clonality: polyclonal  |  Host: Chicken  |  Reactivity: His-tag

330 €
Add to cart
Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
img missing
AS15 2930 | Clonality: monoclonal |  Host: mouse | Reactivity: Polyhistidine (HHHHHH) tagged polypeptide

468 €
Add to cart
Info
img missing
AS15 2937 | Clonality: monoclonal |  Host: Mouse | Reactivity: His-tag (N- or C-terminus label)
468 €
Add to cart
Info
Close