AGO3-PAZ | Argonaute 3 PAZ domain (Chlamydomonas)

Product no: AS14 2799

AS14 2799 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii

Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
302
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated peptide derived from C-terminal of AGO3 protein of Chlamydomonas reinhardtii

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: for reconstitution add 50 l of sterile water
    Storage: store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunoprecipiatation (IP), Western blot (WB)
    Recommended dilution: 1: 2500 (WB)
    Expected | apparent MW: 116,4 | 130 kDa
  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii
  • Application Examples
  • Application example

    Western blot using anti-AGO3 antibodies (Chlamydmonas)
    Total proteins of Chlamydomonas reinhardtii saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-AGO3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (anti-rabbit, DyLight® 800 conjugated, AS12 2460 Agrisera) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

    Courtesy Dr. Betty Chung, Department of Plant Sciences, University of Cambridge, UK
  • Background
  • Background: Argonaute 3(AGO3) of Chlamydmonas reinhardii is cytoplasmic enriched and associated with polysomes and miRNAs (Chung et al. 2019).
  • Product Citations
  • Selected references: Chung et al. (2019) Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression. Sci Rep. 2019 Jul 31;9(1):11091. doi: 10.1038/s41598-019-47415-x.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
Add to cart
Info
img missing
AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 16 €

AS14 2776 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardii

224 €
Add to cart
Info
Close