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AGO3-PAZ | Argonaute 3 PAZ domain (Chlamydomonas)

AS14 2799 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii

AGO3-PAZ | Argonaute 3 PAZ domain (Chlamydomonas)  in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / microRNA at Agrisera AB (Antibodies for research) (AS14 2799)

DATA SHEET IN PDF

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Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated peptide derived from C-terminal of AGO3 protein of Chlamydomonas reinhardtii

Host Rabbit
Clonality Polyclonal
Purity

affinity purified serum in PBS pH 7.4 

Format Lyophilized
Quantity

50 µg

Reconstitution

for reconstitution add 50 µl of sterile water.

Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunoprecipiatation (IP), Western blot (WB)
Recommended dilution 1: 2500 (WB)
Expected | apparent MW

116.4 | 130 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Application examples

Application examples Application example

Western blot using anti-AGO3 antibodies (Chlamydmonas)
Total proteins of Chlamydomonas reinhardtii saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-AGO3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (anti-rabbit, DyLight® 800 conjugated, AS12 2460 Agrisera) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

Courtesy Dr. Betty Chung, Department of Plant Sciences, University of Cambridge, UK

Additional information

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AS10 673 | Anti-AGO9 | argonaute 9, rabbit antibodies

collection of antibodies to micro RNA

Background

Background Argonaute 3(AGO3) of Chlamydmonas reinhardii is cytoplasmic enriched and associated with polysomes and miRNAs (Chung et al. 2019).

Product citations

Selected references Chung et al. (2019) Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression. Sci Rep. 2019 Jul 31;9(1):11091. doi: 10.1038/s41598-019-47415-x.

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