Name:
Phone:
E-mail:
Address:






AGO1 | Argonaute 1 (Chlamydomonas)

AS14 2776 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardii

AGO1 | Argonaute 1 (Chlamydomonas)  in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / plant RNA at Agrisera AB (Antibodies for research) (AS14 2776)
AGO1 | Argonaute 1 (Chlamydomonas)



DATA SHEET IN PDF

Qty: 
224
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

recombinant AGO1 of Chlamydomonas reinhardtii

Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein G purified in PBS pH 7.4.
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 116,4 | 130 kDa

Reactivity

Confirmed reactivity Recombinant AGO1 of Chlamydomonas reinhardtii

Application examples

Application examples Application example

western blot using anti-AGO1 (Chlamydomonas)

Molecular weight markers (1), recombinant AGO1 of Chlamydomonas reinhardtii (2), recombinant AGO1 of Chlamydomonas reinhardtii (1/2 dilution) (3). Recombinant protein was purified using Ni-NTA Agarose (Qiagen), eluted in 50mM NaH2PO4, 500 mM NaCl and 250 mM imidazole (pH 7.5) and diluted in 1xPBS with 2% SDS. Samples were denatured prior to loading with 5 x loading buffer (350 mM Tris-Cl (pH 7.5), 30% glycerol, 10% SDS and 0.6 M DTT) by heating at 95°C for 5 mins. Samples were separated on NuPAGE 7% Tris-Acetate Protein Gel and blotted 1h to PVDF using tank transfer. Blots were blocked with 1xTBS with 0.1% Tween-20 (TBS-T) and 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 15 hrs at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit Ab) diluted to 1:5000 in TBS-T for 1h at RT with agitation. The blot was washed as above and imaged using the Odyssey Infrared Imager and Image Studio v 2.1.

Courtesy of PhD candidate Claire Agius, University of Cambridge, UK

Additional information

Antibody binds to recombinat AGO1 of Chlamydomonas reinhardtii. Endogenous detection remains to be confirmed.

Related products

Background

Background

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Product citations

immunogen:

recombinant AGO1 of Chlamydomonas reinhardtii

Host: Rabbit
Clonality: Polyclonal
Purity: Total IgG. Protein G purified in PBS pH 7.4.
Format: Lyophilized
Quantity: 1 mg
Reconstitution: For reconstitution add 50 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications: Western blot (WB)
recommended dilution: 1 : 1000 (WB)
Expected | apparent MW: 116,4 | 130 kDa
Confirmed reactivity: Recombinant AGO1 of Chlamydomonas reinhardtii
Picture (footer): Application example

western blot using anti-AGO1 (Chlamydomonas)

Molecular weight markers (1), recombinant AGO1 of Chlamydomonas reinhardtii (2), recombinant AGO1 of Chlamydomonas reinhardtii (1/2 dilution) (3). Recombinant protein was purified using Ni-NTA Agarose (Qiagen), eluted in 50mM NaH2PO4, 500 mM NaCl and 250 mM imidazole (pH 7.5) and diluted in 1xPBS with 2% SDS. Samples were denatured prior to loading with 5 x loading buffer (350 mM Tris-Cl (pH 7.5), 30% glycerol, 10% SDS and 0.6 M DTT) by heating at 95°C for 5 mins. Samples were separated on NuPAGE 7% Tris-Acetate Protein Gel and blotted 1h to PVDF using tank transfer. Blots were blocked with 1xTBS with 0.1% Tween-20 (TBS-T) and 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 15 hrs at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit Ab) diluted to 1:5000 in TBS-T for 1h at RT with agitation. The blot was washed as above and imaged using the Odyssey Infrared Imager and Image Studio v 2.1.

Courtesy of PhD candidate Claire Agius, University of Cambridge, UK
additional information (application): Antibody binds to recombinat AGO1 of Chlamydomonas reinhardtii. Endogenous detection remains to be confirmed.
background:

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Related products: AGO1 | Argonaute 1 (Chlamydomonas)

AS14 2797 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
AS14 2799 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii

302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only...
From 26 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €