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AGO1-PAZ | Argonaute 1 PAZ domain (Chlamydomonas)
AS14 2797 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii
- Product Info
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Immunogen: KLH-conjugated peptide derived from AGO1-PAZ domain of Chlamydomonas reinhardtii Cre02.g141050.t1.1
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: for reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1: 500 (WB)
Expected | apparent MW: 109 | 120 kDa
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii
- Application Examples
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Application example 
Total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (goat anti-rabbit IgG (H&L), DyLight® 800 conjugated) 1:2500 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.
Courtesy of Dr. Betty Chung, University of Cambridge, United Kingdom - Additional Information
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Additional information (application): AGO1 signal is detected at higher MW most likely due to post-translational modifications) - Background
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Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection
- Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 % until end of 2023. Use discount code: Conj10
AS14 2776 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardii
AS14 2799 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
AS16 ECL-S-1L, 1L
Choose the appropriate volume in the drop down menu to the right