AGO1-PAZ | Argonaute 1 PAZ domain (Chlamydomonas)

AS14 2797 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii

AGO1-PAZ | Argonaute 1 PAZ domain (Chlamydomonas)

Product Information

Immunogen

KLH-conjugated peptide derived from AGO1-PAZ domain of Chlamydomonas reinhardtii Cre02.g141050.t1.1

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution for reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution

1: 500 (WB)

Expected | apparent MW

109 | 120 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Application examples

Application examples Application example

Western blot using anti-AGO1-PAZ antibodies (Chlamydomonas)

Total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (goat anti-rabbit IgG (H&L), DyLight® 800 conjugated) 1:2500 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

Courtesy of Dr. Betty Chung, University of Cambridge, United Kingdom

Additional information

AGO1 signal is detected at higher MW most likely due to post-translational modifications)

Related products

AS14 2799 | Anti-AGO3-PAZ | Argonaute 3 PAZ domain (Chlamydomonas) , rabbit antibodies
AS14 2726 | Anti-AGO1 | Argonaute 1 (Chlamydomonas), rabbit antibodies

collection of antibodies to micro RNA

Background

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).