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AGO1-PAZ | Argonaute 1 PAZ domain (Chlamydomonas)

AS14 2797 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii

15 % discount on this product, until the 31st of December 2022.
Discount code: Micro15

AGO1-PAZ | Argonaute 1 PAZ domain (Chlamydomonas) in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / plant RNA at Agrisera AB (Antibodies for research) (AS14 2797)
AGO1-PAZ | Argonaute 1 PAZ domain (Chlamydomonas)



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Product Information

Immunogen

KLH-conjugated peptide derived from AGO1-PAZ domain of Chlamydomonas reinhardtii  Cre02.g141050.t1.1

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution for reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution

1: 500 (WB)

Expected | apparent MW

109 | 120 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Application examples

Application examples Application example

Western blot using anti-AGO1-PAZ antibodies (Chlamydomonas)

Total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (goat anti-rabbit IgG (H&L), DyLight® 800 conjugated) 1:2500 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

Courtesy of Dr. Betty Chung, University of Cambridge, United Kingdom

Additional information

AGO1 signal is detected at higher MW most likely due to post-translational modifications)

Related products

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AS14 2799 | Anti-AGO3-PAZ | Argonaute 3 PAZ domain (Chlamydomonas) , rabbit antibodies
AS14 2726 | Anti-AGO1 | Argonaute 1 (Chlamydomonas), rabbit antibodies

collection of antibodies to micro RNA

Background

Background

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

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