Anti-AGO1-PAZ | Argonaute 1 PAZ domain (Chlamydomonas)

Name: Anti-AGO1-PAZ | Argonaute 1 PAZ domain (Chlamydomonas)
SKU: AS14 2797
Price: 329 EUR
Availability: InStock

Product no: AS14 2797

AS14 2797 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii

329 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	KLH-conjugated peptide derived from AGO1-PAZ domain of Chlamydomonas reinhardtii Cre02.g141050.t1.1
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	for reconstitution add 50 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1: 500 (WB)
Expected \| apparent MW:	109 \| 120 kDa

Reactivity
Confirmed reactivity: Chlamydomonas reinhardtii
Application Examples

Application example

Western blot using anti-AGO1-PAZ antibodies (Chlamydomonas)

Total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (goat anti-rabbit IgG (H&L), DyLight® 800 conjugated) 1:2500 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

Courtesy of Dr. Betty Chung, University of Cambridge, United Kingdom

Additional Information
Additional information (application): AGO1 signal is detected at higher MW most likely due to post-translational modifications)
Background

Background:

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Posters Collection
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