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AHB2 | Hemoglobin 2

AS13 2745  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

AHB2 | Hemoglobin 2 in the group Antibodies for Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS13 2745)

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Product Information

Immunogen

recombiant Arabidopsis thaliana AHB2 protein, UniProt:O24521, TAIR: AT3G10520

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

17.9 | 17.9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Beta vulgaris, Brassica napus, Gossypium hirsutum, Solanum lycopersicum, Solanum tuberosum

Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-AHB2 antibodie

Reactivity of AHB2 antibodies to recombinant AHB1 and AHB2
ng of recombinant Arabidopsis thaliana AHB1 and AHB2 are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.

Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA  

western blot using anti-AHB2 antibodies

AHB2 expression in stage 1-15 flowers
Indicated protein amounts of Arabidopsis thaliana extracts are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.

Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA  


Additional information

Related products

Background

Background

AHB2 (Arabidopsis hemoglobin2) belongs to plant globin family and is a heme, iron and oxygen binding protein. Expressed in rosette leaves and young roots and induced by variety of environmental stresses.

Synonymes: AHB2, ARABIDOPSIS HEMOGLOBIN 2, ARATH GLB2, ATGLB2, GLB2, HAEMOGLOBIN 2, HB2, HEMOGLOBIN 2, NON-SYMBIOTIC HAEMOGLOBIN 2, NSHB2.

Product citations

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