AHB2 | Hemoglobin 2
AS13 2745 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
Reactivity
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Application examples
application example
Reactivity of AHB2 antibodies to recombinant AHB1 and AHB2
ng of recombinant Arabidopsis thaliana AHB1 and AHB2 are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.
Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA
AHB2 expression in stage 1-15 flowers
Indicated protein amounts of Arabidopsis thaliana extracts are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.
Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA
Additional information
Background
AHB2 (Arabidopsis hemoglobin2) belongs to the plant globin family, and is a heme-, iron- and oxygen-binding protein. Expressed in rosette leaves and young roots, and induced by a variety of environmental stresses.
Synonymes: AHB2, ARABIDOPSIS HEMOGLOBIN 2, ARATH GLB2, ATGLB2, GLB2, HAEMOGLOBIN 2, HB2, HEMOGLOBIN 2, NON-SYMBIOTIC HAEMOGLOBIN 2, NSHB2.
Product citations
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