AHB1 | Non-symbiotic hemoglobin 1 (A. thaliana)
AS18 4230 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana AHB1, UniProt: O24520, TAIR: AT2G16060
Host
Rabbit
Clonality
Polyclonal
Purity
Antigen affinity purified serum, in PBS pH 7.4.
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl, of sterile water.
Storage
Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples

90 µg/well of total protein extracted freshly from Arabidopsis thaliana seedlings with extraction buffer (100mM Tris-HCl, 150mM NaCl, 0.25% NP-40) containing 1mM PMSF and 1X cOmplete® EDTA-free proteases inhibitors, Sigma) and denatured with buffer (Tris-HCl 0,25 M, pH 6,8; SDS 8% (w/v); glicerol 40% (v/v); β-Mercaptoethanol 20% (v/v) and Bromophenol Blue) at 90°C for 10 min were separated on 12% SDS-PAGE and blotted (20min; 2A; 25V) to PVDF (pore size of 0.2 µm), using semi-dry transfer. Blot was blocked with 5% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 ON/4°C with agitation. The antibody solution was decanted and the blot was washed 2 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (Goat anti-rabbit IgG HRP peroxidase conjugated, AS09 602) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed 4 times for 5 min in TBS-T at RT with agitation and developed with Agrisera ECL SuperBright, AS16 ECL-S. Exposure time was 5 seconds.
The lines used in the experiments were generated by:
-Hebesltrup et al., 2008 corresponds to 35S: GFP-PGB1
-Perazzolli et al., 2004 corresponds to 35S:PGB1
Courtesy of Dr. Inmaculada Sánchez-Vicente and Isabel Manrique-Gil, USAL-CIALE Salamanca, Spain

90 µg/well of total protein extracted freshly from Arabidopsis thaliana seedlings with extraction buffer (100mM Tris-HCl, 150mM NaCl, 0.25% NP-40) containing 1mM PMSF and 1X cOmplete® EDTA-free proteases inhibitors, Sigma) and denatured with buffer (Tris-HCl 0,25 M, pH 6,8; SDS 8% (w/v); glicerol 40% (v/v); β-Mercaptoethanol 20% (v/v) and Bromophenol Blue) at 90°C for 10 min were separated on 12% SDS-PAGE and blotted (20min; 2A; 25V) to PVDF (pore size of 0.2 µm), using semi-dry transfer. Blot was blocked with 5% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 ON/4°C with agitation. The antibody solution was decanted and the blot was washed 2 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (Goat anti-rabbit IgG HRP peroxidase conjugated, AS09 602) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed 4 times for 5 min in TBS-T at RT with agitation and developed with Agrisera ECL SuperBright, AS16 ECL-S. Exposure time was 5 seconds.
The lines used in the experiments were generated by:
-Hebesltrup et al., 2008 corresponds to 35S: GFP-PGB1
-Perazzolli et al., 2004 corresponds to 35S:PGB1
Courtesy of Dr. Inmaculada Sánchez-Vicente and Isabel Manrique-Gil, USAL-CIALE Salamanca, Spain
Additional information
Conditions for detection of endogenous AHB1 remain to be determined.
Background
Background
AHB1 (Non-symbiotic hemoglobin 1) is involved in a cellular response to hypoxia and displays oxygen carrier activity. Alternative names: ARAth, GLB1, Hb1AHB1, ARATH GLB1, ATGLB1, CLASS I HEMOGLOBIN, GLB1, HB1, HEMOGLOBIN 1, NSHB1, PGB1, PHYTOGLOBIN 1.
Product citations
Related products: AHB1 | Non-symbiotic hemoglobin 1 (A. thaliana)
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 26 €
AS13 2745 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabi...
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 ...
10 ...
201 €