AKINB3 | SNF1-related protein kinase regulatory subunit beta-3
AS15 2859 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta 3 sequence, UniProt: Q9ZUU8, TAIR: AT2G28060
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
12,7 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Brasica rapa, Capsella rubella, Ricinus communis, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example
Recombinant AKIN beta 3 were separated by 10% SDS-PAGE and transferred to a PVDF membrane using semi-dry blotting (for 1h). Blots were blocked with 5% low-fat milk powder in TBS-T (0.01% Tween 20) for 1h at room temperature (RT) with agitation. The blot was then incubated overnight at 4 °C in the primary antibody at a dilution of 1: 1 000 in 1% low-fat milk powder in TBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in TBS-T at RT with agitation. The blot was then incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in 1% low-fat milk powder in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min and developed for 2 min with chemiluminescence detection reagent in low picogram range, according to the manufacturer's instructions. Exposure time to film was 10 min.
Courtesy of PhD student Sander Hulsmans, KU Leuven, Belgium
Recombinant AKIN beta 3 were separated by 10% SDS-PAGE and transferred to a PVDF membrane using semi-dry blotting (for 1h). Blots were blocked with 5% low-fat milk powder in TBS-T (0.01% Tween 20) for 1h at room temperature (RT) with agitation. The blot was then incubated overnight at 4 °C in the primary antibody at a dilution of 1: 1 000 in 1% low-fat milk powder in TBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in TBS-T at RT with agitation. The blot was then incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in 1% low-fat milk powder in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min and developed for 2 min with chemiluminescence detection reagent in low picogram range, according to the manufacturer's instructions. Exposure time to film was 10 min.
Courtesy of PhD student Sander Hulsmans, KU Leuven, Belgium
Additional information
Background
Background
AKIN ß3 (SNF1-related protein kinase regulatory subunit beta-3) is a regulatory subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product citations
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