showForm anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1 antibodies

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AKINB1 | SNF1-related protein kinase regulatory subunit beta-1

AS09 460 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

AKINB1 | SNF1-related protein kinase regulatory subunit beta-1 in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS09 460)

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Product Information

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-1 Q84VQ1, At5g21170

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 2 µg/ml
Expected | apparent MW

30.7 | 35 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in Zea mays

Application examples

Application examples

Application example

western blot detection using anti-AKIN beta-1 antibodies

25 µg of empty vector (1), pCDNA 3.1 – HA- tagged AKIN-beta1 (2), pCDNA 3.1 – HA- tagged AKIN-beta2 (3), 50 µg of total protein from Arabidopsis thaliana leaves had been homogenized into 50 mM NaPO4 pH 7.5, 20 mM KCl, 0.5 M sucrose, 0.2 mM PMSF, 10 mM DTT and protease inhibitor cocktail (Sigma, P9599). Extracts were separared on 10%NuPage Bis-Tris Novex SDS PAGE (Invitrogen) gels followed by a transfer for 60 min to PVDF membrane. Filters were blocked for 1h with 5% low-fat milk powder in PBS-T (0.1% TWEEN 20) and probed with anti-AKINß 1 antibodies (2 µg/ml for 60 min at RT) followed by Protein G-HRP (Bio-Rad; 1:3 000 for 60 min at RT) in PBS-T. Antibody incubations were followed by washings in PBS-T (3 x 5 min). All washing steps were performed at RT with agitation. Signal was detected with ECL (Millipore) using CCD camera. Exposure time was 10 seconds.

Courtesy Dr. David Stapleton, Melbourne, Australia

Additional information

Related products

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collection of antibodies to proteins involved in signal transduction

Plant protein extraction buffer

Secondary antibodies

Background

Background

AKIN beta-1 (AKINB1) is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.

Product citations

Selected references Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.

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