Anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1

Product no: AS09 460

AS09 460 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-1 Q84VQ1, At5g21170

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 200 µg
    Reconstitution: For reconstitution add 200 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 2 µg/ml
    Expected | apparent MW: 30.7 | 35 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity:

    Arabidopsis thaliana

    Not reactive in: Zea mays
  • Application Examples
  • Application example

    western blot detection using anti-AKIN beta-1 antibodies

    25 µg of empty vector (1), pCDNA 3.1 – HA- tagged AKIN-beta1 (2), pCDNA 3.1 – HA- tagged AKIN-beta2 (3), 50 µg of total protein from Arabidopsis thaliana leaves had been homogenized into 50 mM NaPO4 pH 7.5, 20 mM KCl, 0.5 M sucrose, 0.2 mM PMSF, 10 mM DTT and protease inhibitor cocktail (Sigma, P9599). Extracts were separared on 10%NuPage Bis-Tris Novex SDS PAGE (Invitrogen) gels followed by a transfer for 60 min to PVDF membrane. Filters were blocked for 1h with 5% low-fat milk powder in PBS-T (0.1% TWEEN 20) and probed with anti-AKINß 1 antibodies (2 µg/ml for 60 min at RT) followed by Protein G-HRP (Bio-Rad; 1:3 000 for 60 min at RT) in PBS-T. Antibody incubations were followed by washings in PBS-T (3 x 5 min). All washing steps were performed at RT with agitation. Signal was detected with ECL (Millipore) using CCD camera. Exposure time was 10 seconds.

    Courtesy Dr. David Stapleton, Melbourne, Australia

  • Background
  • Background:

    AKIN beta-1 (AKINB1) is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.

  • Product Citations
  • Selected references: Belda-Palazón et al. (2020) A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth. Nat Plants. 2020 Nov;6(11):1345-1353. doi: 10.1038/s41477-020-00778-w. Epub 2020 Oct 19. PMID: 33077877.
    Crozet et al. (2016). SUMOylation represses SnRK1 signaling in Arabidopsis. Plant J. 2016 Jan;85(1):120-133. doi: 10.1111/tpj.13096.
    Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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