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ALEU | Thiol protease aleurain

AS20 4406 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana
ALEU | Thiol protease aleurain in the group Antibodies Plant/Algal  / Nitrogen Metabolism at Agrisera AB (Antibodies for research) (AS20 4406)
ALEU | Thiol protease aleurain



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Product Information

Immunogen Recombinant, His6-tagged, ALEU protein from Arabidopsis thaliana, UniProt: Q8H166, TAIR: At5g60360
Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format Liquid at 2 mg/ml.
Quantity 100 ĩg
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1: 1000 - 1: 4000 (WB)
Expected | apparent MW 38,9 | 24 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica oleracea, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-ALEU antibodies

Arabidopsis thaliana crude extract was prepared from 7 day-old seedlings (1) 19 day-old seedlings (2) which were freshly extracted to a crude extract with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. were separated on 15-20 % SDS-PAGE and blotted to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.

The ALEU protein is synthesized as preproprotein with 39 kDa, the signal peptide of 21 amino acids is removed and the propeptide of N-terminal 119 amino acids are removed and not present in the mature, functional protein. The size of apparent molecular mass analyzed by Western blot is therefore 24 kDa.

Additional information

Related products

Background

Background Aleu protein may play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation. Catalytic activity : Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase. Cellular localisation: vacuole. 

Alternative names: Senescence-associated gene product 2

Product citations

Selected references Takagi et al. et al. (2013). MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell. 2013 Nov;25(11):4658-75. doi: 10.1105/tpc.113.118158. (Western blot, Arabidopsis thaliana)
Ueda et al. (2006). AtVAM3 is required for normal specification of idioblasts, myrosin cells. Plant Cell Physiol. 2006 Jan;47(1):164-75. doi: 10.1093/pcp/pci232. (Western blot, Arabidopsis thaliana)

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