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Anti-DMC1 | Disruption of meiotic control 1
AS23 4964 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated peptide derived from protein sequence of DMC1 of Arabidopsis thaliana, UniProt: Q39009 GeneID: At3g22880
Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Chromatin immunoprecipitation (ChIP), Western blot (WB) Recommended dilution: 1 : 5000 (WB) Expected | apparent MW: 38 kDa - Reactivity
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Confirmed reactivity: Triticum aestivum Predicted reactivity: Arabidopsis thaliana, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Solanum lycopresicum, Solanum tuberosum, Zea mays.
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Samples:
pIXHalo - negative control
pIX-Halo-DMC1 - overexpressed DMC1 with Halo tag
Different wheat (Triticum aestivum) varieties
L: Leaves (negative control)
Sp: Spike
Expected size for recombinant DMC1-Halo protein: 72 kDa
Expected size for DMC1 protein: 38 kDa5 µg/well of nuclear protein extracted freshly from wheat cultivars leaves (L) and immature spikes (S). Exact buffer components were: laemmli 4X, DTT 10X and denatured with exact buffer components at 90 °C 5 min. Samples were separated in the cold on 4-12 % NuPAGE and blotted for 7 min iBlot™ 2 Transfer Stacks, nitrocellulose (pore size of 0.2 um), using: dry transfer in the cold. Blot was blocked with StartingBlock™ Blocking Buffer for: 30 min RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h/RT with agitation in TBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 20 000 in for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: SuperSignal™ West Pico PLUS Chemiluminescent Substrate and Thermoscientific. Exposure time was minutes.
Note: background signal can be decreased by increasing the length of blocking time to ON/4C.Dr. Floriane Cheron, INRAE UMR1095, France
- Additional Information
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Additional information (application): DMC1 protein is only expressed in meiotic cells. Total cell extract from leaves can be used as a negative control. - Background
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Background: DMC1 (Disruption of meiotic control) is a meiosis-specific recombinase, acting as the primary catalyst for homologous recombination and DNA double-strand break (DSB) repair during meiosis. It facilitates pairing and synapsis of homologous chromosomes, ensuring accurate segregation and fertility by promoting interhomolog repair over intersister repair. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)