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Anti-NRPE1 | DNA-directed RNA polymerase V subunit 1
AS23 4998 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana NRPE1 protein sequence, UniProt: Q5D869 TAIR: AT2G40030 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Immunofluorescence (IF) Recommended dilution: 1 : 250 (IF) Expected | apparent MW: 218 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Brassica napus, Capsella rubella, Camelina sativa, Populus tomentosa
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Type of material: Arabidopsis thaliana 7-day-old seedlings, WT (Col0) and in nrpe1 mutant (SALK_029919)Fixation: formaldehyde
Hydrophilization: no
Cell wall digestion: yes
Membrane permeabilization: Triton at 0.5%
Antigen retrieval: no
Blocking buffer: BSA 0.3%
Washing buffer: PBS
Primary antibody dilution and incubation time: 1:250 ON/4°C
Secondary antibody: 1:1000 1h/RT, INVITROGEN A21430 goat anti-rabbit Alexa Fluor 555
Co-staining of the nucleus (DAPI): yesCourtesy of Dr. Lauriane Simon, iGReD, France
- Background
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Background: NRPE1 (DNA-directed RNA polymerase V subunit 1) is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase V involved in RNA-directed DNA methylation-dependent (RdDM) silencing of endogenous repeated sequences, including transposable elements. Alternative protein names:
DNA-directed RNA polymerase D subunit 1b (AtNRPD1b; Nuclear RNA polymerase D 1b)
DNA-directed RNA polymerase E subunit 1 (Nuclear RNA polymerase E 1)
Protein DEFECTIVE IN MERISTEM SILENCING 5 Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 3 Protein RNA-DIRECTED DNA METHYLATION DEFECTIVE 1 RNA polymerase IV subunit 1 (POL IV 1) - Product Citations
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Selected references: 38221900 - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
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