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Anti-RFa1/Rpa1 | Replication factor protein 1 (S.cerevisiae)
Product no: AS26 5139
AS26 5139 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces cerevisiae
352
€
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- Product Info
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Immunogen: Recombinant Rfa1 protein from Saccharomyces cerevisiae, UniProt: P22336 Host: Rabbit Clonality: Polyclonal Purity: Purified IgG fraction with protein A from rabbit antiserum Quantity: 100 µg Storage: Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunooprecipitation (IP), Western blot (WB) Recommended dilution: 1: 200 (IP), 1 : 2000 (WB) Expected | apparent MW: 70.3 kDa - Reactivity
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Confirmed reactivity: Saccharomyces cerevisiae Predicted reactivity: Species of your interest not listed? Contact us - Application Examples
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The extract of strain BY4741 (20μg) was separated on SDS-PAGE 12% gel. The anti-Rfa1 antibody was used at 1:2 000 dilution (62-150 antiserum) and 1μg/ml (IgG purified). As the second antibody was used at 1:10 000 dilution. - Additional Information
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Additional information: Concentration 1 mg/ml in PBS with 50% glycerol. - Background
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Background: Rfa1 (Replication Factor A protein 1) as part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication. - Reviews:
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