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Anti-SMC-1 | Structural maintenance of chromosomes protein 1
Product no: AS22 4824
AS22 4824 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
329
€
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- Product Info
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Immunogen: KLH-conjugated peptide derived from SMC-1 of Arabidopsis thaliana, UniProt: Q6Q1P4, GeneID: AT3G54670 Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Immunofluorescence (IF) Recommended dilution: 1 : 100 (IF) Expected | apparent MW: 140 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Species of your interest not listed? Contact us Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Type of material: Fresh flowers were dissected and digested with an enzyme mix to get isolated cells, which afterwards were treated with a detergent to dissolve the nuclear envelope, and then the chromatin was fixed in paraformaldehyde 4%.
Fixation: formaldehyde (paraformaldehyde 4% pH 8) X | Methanol, 37°C
Hydrophilization: no
Cell wall digestion: yes
Membrane permeabilization: DMSO-IGEPA, enzyme digestion Blocking buffer: BSA 3% in PBST (Triton 1%)
Washing buffer: PBST (Triton 1%)
Primary antibody dilution and incubation time: 1:200 in BSA 3% in PBST overnight at 4°C
Secondary antibody: goat anti-rabbit IgG, TRTC-conjugated, 1:100 in BSA 3% in PBST for 90 min at room temperature
Co-staining of the nucleus (DAPI): yes
Courtesy of Dr. Nadia Fernández Jiménez, Universidad Complutense de Madrid (UCM), Spain
- Background
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Background: SMC-1 (Structural maintenance of chromosomes protein 1) is the central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement, and it is also essential protein for plant viability, which is required for chromosome segregation (e.g. sister chromatid alignment) and cell division during embryogenesis. Alternative protein names: Chromosome segregation protein SMC-1 Cohesin complex subunit SMC-1 Protein TITAN8
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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