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Arabinogalactan-4 (clone CCRC-M78)

AS16 3231-1ml  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Lactuca sativa, Sinapus sp., Solanum lycopersicum

From the laboratory of Michael G. Hahn, PhD, University of Georgia

Arabinogalactan-4 (clone CCRC-M78) in the group Antibodies Plant/Algal / Cell Wall / Dr. Hahn collection, University of Georgia at Agrisera AB (Antibodies for research) (AS16 3231-1ml)
Arabinogalactan-4 (clone CCRC-M78)



DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen MeBSA-conjugated Arabidopsis thaliana rhamnogalacturonan-I
Host Mouse
Clonality Monoclonal
Subclass/isotype IgM
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution Undiluted or at 1 : 10 (ELISA), (IF), (IHC)

Reactivity

Confirmed reactivity Acer pseudoplatanus, Arabidopsis thaliana, Lactuca sativa, Sinapus sp., Solanum lycopersicum
Predicted reactivity Gum, sycamore, tomato, lettuce and sinapus glycan group of arabinogalactan-4
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Exact working dilution needs to be determined by end user

Related products

Background

Background Arabinogalactans are polymers composed of arabinose and galactose monosaccharides. They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones. When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule. AGP also functions as a glue for sealing wounds in plants.  
Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products.

This pectic polysaccharide antibody belongs to the arabinogalactan-4 group of antibodies. Pectin consists of polysaccharides found in the primary cell wall of most plants which binds cells together in the middle lamella.

Product citations

Selected references Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
Clonality: Monoclonal
Format: Liquid
Host: Mouse
immunogen: MeBSA-conjugated Arabidopsis thaliana rhamnogalacturonan-I
Purity: Cell culture supernatant.
Quantity: 1 ml
recommended dilution: Undiluted or at 1 : 10 (ELISA), (IF), (IHC)
storage: Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class: IgM
tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
All references: Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
background: Arabinogalactans are polymers composed of arabinose and galactose monosaccharides. They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones. When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule. AGP also functions as a glue for sealing wounds in plants.  
Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products.

This pectic polysaccharide antibody belongs to the arabinogalactan-4 group of antibodies. Pectin consists of polysaccharides found in the primary cell wall of most plants which binds cells together in the middle lamella.
additional information: Exact working dilution needs to be determined by end user
Confirmed reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Lactuca sativa, Sinapus sp., Solanum lycopersicum
predicted reactivity: Gum, sycamore, tomato, lettuce and sinapus glycan group of arabinogalactan-4
not reactive in: No confirmed exceptions from predicted reactivity are currently known