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ATG1a | Serine/threonine-protein kinase ATG1a

AS19 4274  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

ATG1a | Serine/threonine-protein kinase ATG1a in the group Antibodies Plant/Algal  / Protein Modifications / Autophagy-related and Ubiquitin-like Proteins at Agrisera AB (Antibodies for research) (AS19 4274)
ATG1a | Serine/threonine-protein kinase ATG1a



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Product Information

Immunogen Full length, N-terminally HIS-tagged ATG1a of Arabidopsis thaliana   protein sequence UniProt: Q94C95, TAIR: At3g61960, overexpressed in E.coli and injected as a gel piece
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 69,6 | 70 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Noccaea caerulescens
Species of your interest not listed? Contact us
Not reactive in Oryza sativa

Application examples

Additional information

Antibody may also recognise ATG1b and ATG1c; does not recognise ATG1t, Suttangkakul et al. (2011).

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Background

Background ATG1a (Serine/threonine-protein kinase ATG1a) is a serine/threonine protein kinase involved in autophagy in a nutritional condition-dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Becomes a target of autophagy under nutrient starvation and connects autophagy to plant nutritional status.

Alternative names: Autophagy-related protein 1a, AtAPG1a.

Product citations

Selected references Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.
immunogen: Full length, N-terminally HIS-tagged ATG1a of Arabidopsis thaliana   protein sequence UniProt: Q94C95, TAIR: At3g61960, overexpressed in E.coli and injected as a gel piece
Host:

Rabbit

Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 50 µl
Reconstitution: For reconstitution add 50 µl, of sterile water
storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications: Western blot (WB)
recommended dilution: 1 : 1000 (WB)
Expected | apparent MW: 69,6 | 70 kDa
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Noccaea caerulescens
Species of your interest not listed? Contact us
not reactive in: Oryza sativa
additional information (application): Antibody may also recognise ATG1b and ATG1c; does not recognise ATG1t, Suttangkakul et al. (2011).
background: ATG1a (Serine/threonine-protein kinase ATG1a) is a serine/threonine protein kinase involved in autophagy in a nutritional condition-dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Becomes a target of autophagy under nutrient starvation and connects autophagy to plant nutritional status.

Alternative names: Autophagy-related protein 1a, AtAPG1a.
All references: Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.

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