ATG12b | Autophagy-related protein 12b
AS19 4278 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Protocols | Customer reviews |
Product Information
Immunogen
Recombinant, full length ATG12b ofArabidopsis thaliana protein sequence UniProt: Q9LVK3-1, TAIR: At3g13970
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 50 ĩl, of sterile water
Storage
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 3000 (WB)
Expected | apparent MW
10,4 | 12 and 50 kDa in wilde type plants and 12 kDa in some autophagy deficient mutants
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
This antibody may also recognise ATG12a; in wild-type, ATG12 is also conjugated to ATG5
Background
Background
ATG12b (Autophagy-related protein 12b) is an Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation.
Alternative names: Ubiquitin-like protein ATG12B, APG12-like protein b, AtAPG12b.
Alternative names: Ubiquitin-like protein ATG12B, APG12-like protein b, AtAPG12b.
Product citations
Selected references
Chung et al. (2010). ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci. Plant J. 2010 May;62(3):483-93. doi: 10.1111/j.1365-313X.2010.04166.x.
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