ATG12b | Autophagy-related protein 12b

Product no: AS19 4278

AS19 4278  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: Recombinant, full length ATG12b ofArabidopsis thaliana   protein sequence UniProt: Q9LVK3-1, TAIR: At3g13970
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C (short tem, months) or at -80C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW: 10,4 | 12 and 50 kDa in wilde type plants and 12 kDa in some autophagy deficient mutants
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information (application): This antibody may also recognise ATG12a; in wild-type, ATG12 is also conjugated to ATG5
  • Background
  • Background: ATG12b (Autophagy-related protein 12b) is an Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation.

    Alternative names: Ubiquitin-like protein ATG12B, APG12-like protein b, AtAPG12b.
  • Product Citations
  • Selected references: Chung et al. (2010). ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci. Plant J. 2010 May;62(3):483-93. doi: 10.1111/j.1365-313X.2010.04166.x.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
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    Number of reviews: (1)
    Jose Julian | 2021-05-18
    0 | 0
    We were able to detect the correct protein thanks to this antibody but it also presented quite abundant background.

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AS19 4276  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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