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ATG12b | Autophagy-related protein 12b

AS19 4278  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

ATG12b | Autophagy-related protein 12b in the group Antibodies for Plant/Algal  / Protein Modifications / Autophagy-related and Ubiquitin-like Proteins at Agrisera AB (Antibodies for research) (AS19 4278)

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Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen Recombinant, full length ATG12b ofArabidopsis thaliana   protein sequence UniProt: Q9LVK3-1, TAIR: At3g13970
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 10.4 | 12 and 50 kDa in wilde type plants and 12 kDa in some autophagy deficient mutants

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody may also recognise ATG12a; in wild-type. ATG12 is also conjugated to ATG5.

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Background

Background ATG12b (Autophagy-related protein 12b) is an Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation.

Alternative names: Ubiquitin-like protein ATG12B, APG12-like protein b, AtAPG12b.

Product citations

Selected references Chung et al. (2010). ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci. Plant J. 2010 May;62(3):483-93. doi: 10.1111/j.1365-313X.2010.04166.x.

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