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ATG7 | Autophagy-related protein 7

AS19 4277  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

ATG7 | Autophagy-related protein 7 in the group Antibodies for Plant/Algal  / Protein Modifications / Autophagy-related and Ubiquitin-like Proteins at Agrisera AB (Antibodies for research) (AS19 4277)

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Product Information

Immunogen Recombinant ATG7 of Arabidopsis thaliana, UniProt: Q94CD5-1, TAIR: At5g45900, overexpressed in E.coli, collected in inclusion bodies, which were solubilized before immunization
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 76.5 | 75 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica rapa, Capsella rubella , Citrus clementina, Noccaea caerulescens
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Protein name was changed from APG7 to ATG7 after Doelling paper was published.

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AS15 2831 | Anti-ATG4 | Autophagy protein 4, rabbit antibodies
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AS14 2811 | Anti-ATG8A | Autophagy-related protein 8a, rabbit antibodies
AS14 2769PRE | ATG8 | Autophagy-related protein, pre-immune serum

AS14 2769 | Anti-ATG8 | Autophagy-related protein, rabbit antibodies

AS16 4071 | Anti-ATG9 | Autophagy-related protein 9 (N-terminal), rabbit antibodies
AS16 4072 | Anti-ATG9 | Autophagy-related protein 9 (C-terminal), rabbit antibodies

AS19 4278 | Anti-ATG12b | Autophagy-related protein 12b, rabbit antibodies
AS19 4279 | Anti-ATG13a | Autophagy-related protein 13a, rabbit antibodies
AS19 4280 | Anti-ATG16 | Autophagy-related protein 16, rabbit antibodies

AS14 2805 | Anti-NBR1 | Autophagy substrate NBR1, rabbit antibodies
AS19 4281 | Anti-NBR1 | Autophagy substrate NBR1, rabbit antibodies

Background

Background ATG7 (Autophagy-related protein 7) is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Acts in the senescence process, degradation of damaged peroxisomes and non-selective degradation of chlorophylls and photosynthetic proteins during stress-induced leaf yellowing.

Alternative names: Ubiquitin-like modifier-activating enzyme atg7, ATG12-activating enzyme E1 atg7, AtAPG7, Protein PEROXISOME UNUSUAL POSITIONING 4.

Product citations

Selected references Doelling et al. (2002). The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana. J Biol Chem. 2002 Sep 6;277(36):33105-14.

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