ATG5 | Autophagy protein 5

Product no: AS19 4276

AS19 4276  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: Full length, recombinant ATG5 of Arabidopsis thaliana   protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E.coli
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW: 38,5 | 50 kDa (in wilde type plants) and ca, 38 kDa in autophagy defective mutants
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Solanum lycopersicum, Solanum tuberosum
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information (application): In wild-type plants almost all ATG5 is conjugated to ATG12.

    This item can be sold containing ProClin
  • Background
  • Background: ATG5 (Autophagy protein 5) is a protein required for autophagy. Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants and a complete proteolysis of chloroplast stroma proteins in senescent leaves, in the degradation of damaged peroxisomes.

    Alternative names: Protein autophagy 5, AtAPG5.
  • Product Citations
  • Selected references: Thompson et al. (2005). Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways. Plant Physiol. 2005 Aug;138(4):2097-110.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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AS19 4275  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS19 4276  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

302 €
Add to cart
Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
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