ATG5 | Autophagy protein 5
AS19 4276 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
Immunogen
Full length, recombinant ATG5 of Arabidopsis thaliana protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E.coli
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 50 ĩl, of sterile water
Storage
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 3000 (WB)
Expected | apparent MW
38,5 | 50 kDa (in wilde type plants) and ca, 38 kDa in autophagy defective mutants
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Solanum lycopersicum, Solanum tuberosum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
In wild-type plants almost all ATG5 is conjugated to ATG12.
This item can be sold containing ProClin
This item can be sold containing ProClin
Background
Background
ATG5 (Autophagy protein 5) is a protein required for autophagy. Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants and a complete proteolysis of chloroplast stroma proteins in senescent leaves, in the degradation of damaged peroxisomes.
Alternative names: Protein autophagy 5, AtAPG5.
Alternative names: Protein autophagy 5, AtAPG5.
Product citations
Selected references
Thompson et al. (2005). Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways. Plant Physiol. 2005 Aug;138(4):2097-110.
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