ATG5 | Autophagy protein 5

Name: ATG5 | Autophagy protein 5
SKU: AS19 4276
Price: 302 EUR
Availability: InStock

AS19 4276 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

ATG5 | Autophagy protein 5 in the group Antibodies Plant/Algal / Protein Modifications / Autophagy-related and Ubiquitin-like Proteins at Agrisera AB (Antibodies for research) (AS19 4276)

Product Information

Immunogen Full length, recombinant ATG5 of Arabidopsis thaliana protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E.coli

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW 38,5 | 50 kDa (in wilde type plants) and ca, 38 kDa in autophagy defective mutants

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Solanum lycopersicum, Solanum tuberosum

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

In wild-type plants almost all ATG5 is conjugated to ATG12.

This item can be sold containing ProClin

Background

Background ATG5 (Autophagy protein 5) is a protein required for autophagy. Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants and a complete proteolysis of chloroplast stroma proteins in senescent leaves, in the degradation of damaged peroxisomes.

Alternative names: Protein autophagy 5, AtAPG5.

Product citations

Selected references Thompson et al. (2005). Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways. Plant Physiol. 2005 Aug;138(4):2097-110.

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immunogen:	Full length, recombinant ATG5 of Arabidopsis thaliana protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E.coli
Reconstitution:	For reconstitution add 50 µl, of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 3000 (WB)
calculated \| apparent molecular mass [kDa]:	38,5 \| 50 kDa (in wilde type plants) and ca, 38 kDa in autophagy defective mutants
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Solanum lycopersicum, Solanum tuberosum
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information (application):	In wild-type plants almost all ATG5 is conjugated to ATG12. This item can be sold containing ProClin
background:	ATG5 (Autophagy protein 5) is a protein required for autophagy. Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants and a complete proteolysis of chloroplast stroma proteins in senescent leaves, in the degradation of damaged peroxisomes. Alternative names: Protein autophagy 5, AtAPG5.
All references:	Thompson et al. (2005). Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways. Plant Physiol. 2005 Aug;138(4):2097-110.