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Anti-Avi Tag Monoclonal Antibody
- Product Info
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Immunogen: Recombinant protein containing HIS+AVI tag sequence: GLNDIFEAQKIEWHE Sub class: IgG Host: Mouse Clonality: Monoclonal Purity: Affinity purified in PBS pH 7.4 and 50 % glycerol. Contains 0.02 % sodium azide. Format: Liquid Quantity: 50 µg Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.Tested applications: ELISA (ELISA), Western Blot (WB) Recommended dilution: 1:500-1:5000 (WB) Expected | apparent MW: Depends upon MW of fusion partner - Reactivity
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Confirmed reactivity: AVI - Application Examples
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3 µg of AVI-recombinant protein was loaded/well and separated on Tris-Glycine 12 % SDS-PAGE in reduced conditions at 40 mA followed by a semi-dry transfer at 60 mA for 80 minutes (0.45mm PVDF membrane purchased from Millipore). Blocking was done with 5% skim milk in PBS at RT/1h. Primary antibody was diluted in 0.05% PBST + 2.5% skim milk powder and incubated ON/4°C in a dilution of 1: 5000. Washing was done with 0.1% PBST (4 times, 10 minutes each) followed by a secondary antibody was diluted to 1:50 000 (0.05% with PBST + 2.5% skim milk powder) and incubated at RT/2h followed by four time washes with 0.1% PBST for 10 minutes each and chemiluminescent reaction development and recording of a signal.
- Additional Information
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Additional information (application): Antibody is present in PBS, pH 7.4, containing 0.5% BSA and 50% Glycerol. Contains 0.02% sodium azide as preservative. - Background
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Background: Avi-tag monoclonal antibody is designed to specifically recognize and bind to the Avi-tag peptide. The Avi-tag is a short peptide sequence (GLNDIFEAQKIEWHE) that is biotinylated by the enzyme BirA (biotin ligase). This tag is commonly used in recombinant protein expression to facilitate the detection, purification, and characterization of tagged proteins.
The Avi-tag system is particularly useful because the biotinylation of the Avi-tag allows for strong and specific interactions with streptavidin or avidin, which can be exploited in various biochemical and molecular biology techniques.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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Accessories
AS22 4822 | Clonality: Polyclonal| Host: Rabbit | Reactivity: Avi-Tag
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