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Anti-Avi-tag
AS22 4822 | Clonality: Polyclonal| Host: Rabbit | Reactivity: Avi-Tag tagged proteins
- Product Info
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Immunogen: KLH-conjugated Avi tag peptide GLNDIFEAQKIEWHE Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: ELISA (ELISA) Recommended dilution: 1 : 3000 (ELISA) Expected | apparent MW: Depends upon fusion partner - Reactivity
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Confirmed reactivity: Avi tag - Additional Information
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Additional information (application): Reactivity of this antibody on Avi tagged recombinant proteins remains to be determined. - Background
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Background: Avi Tag is a type of enzymatic biotinylation tag (short peptide tag) This tag is specifically recognized and biotinylated by the E. coli biotin ligase enzyme BirA. After biotinylation, the tag can be used for: Affinity purification (via streptavidin/avidin beads) Immobilization on surfaces Protein–protein interaction assays Imaging applications (using streptavidin-fluorophore conjugates). - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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