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Anti-E2 Tag Monoclonal Antibody
Product no: AS25 5102
AS25 5102 | Clonality: Monoclonal | Host: Mouse | Reactivity: E2-tagged proteins
169
€
Customer reviews
Delivery:
3-6 business days
- Product Info
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Immunogen: Synthetic peptide SSTSSDFRDR derived from E2 protein sequence Sub class: IgG Host: Mouse Clonality: Monoclonal Purity: Affinity purified in PBS pH 7.4 and 50 % glycerol. Contains 0.02 % sodium azide. Format: Liquid Quantity: 50 µg Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1:500-1:5000 (WB) Expected | apparent MW: Depends upon MW of fusion partner - Reactivity
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Confirmed reactivity: E2 tagged proteins - Application Examples
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2 μg of E2 fusion protein/well was subjected to Tris-Glycine 12 % SDS-PAGE gel electrophoresis in reduced conditions at 40 mA followed by a semi-dry transfer at 60 mA for 80 minutes (0.45mm PVDF membrane purchased from Millipore). Blocking was done with 5% skim milk in PBS at RT/1h. Primary antibody was diluted in 0.05% PBST + 2.5% skim milk powder and incubated ON/4°C in a dilution of 1: 5000 (1) and 1: 10 000 (2) . Washing was done with 0.1% PBST (4 times, 10 minutes each) followed by a secondary antibody was diluted to 1:10 000 (0.05% with PBST + 2.5% skim milk powder) and incubated at RT/2h followed by four time washes with 0.1% PBST for 10 minutes each and chemiluminescent reaction development and recording of a signal.
- Additional Information
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Additional information (application): Antibody is present in PBS, pH 7.4, containing 0.5% BSA and 50% Glycerol. Contains 0.02% sodium azide as preservative. - Background
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Background: E2 tag is a small peptide sequence derived from the E2 protein of the bovine papillomavirus. It is used as an epitope tag in recombinant protein expression systems to facilitate the detection and purification of tagged proteins, including antibodies. Monoclonal antibodies against the E2 tag provide high specificity and affinity for the E2-tagged proteins. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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