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Anti-E2 Tag (monoclonal, clone 24F4)
- Product Info
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Immunogen: Synthetic peptide dervied from E2 protein sequence Sub class: IgG Host: Mouse Clonality: Monoclonal Purity: Affinity purified in PBS pH 7.4 and 50 % glycerol. Contains 0.02 % sodium azide. Format: Liquid Quantity: 50 µg Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1:500-1:5000 (WB) Expected | apparent MW: Depends upon MW of fusion partner - Reactivity
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Confirmed reactivity: E2 tagged proteins - Application Examples
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5 and 2 μg E2 fusion protein/well.
Primary antibody at dilution 1: 2000. - Additional Information
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Additional information (application): Antibody is present in PBS, pH 7.4, containing 0.5% BSA and 50% Glycerol. Contains 0.02% sodium azide as preservative. - Background
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Background: E2 tag is a small peptide sequence derived from the E2 protein of the bovine papillomavirus. It is used as an epitope tag in recombinant protein expression systems to facilitate the detection and purification of tagged proteins, including antibodies. Monoclonal antibodies against the E2 tag provide high specificity and affinity for the E2-tagged proteins. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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