BAR | Phosphinothricin N-acetyltransferase (36-50)

Product no: AS21 4613

AS21 4613   | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: BAR (BASTA) epitope tag (36-50)

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from position 36-50 of Phosphinothricin N-acetyltransferase (BAR or BASTA), UniProt: P16426
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 20.6 kDa
  • Reactivity
  • Confirmed reactivity: BAR (BASTA)
    Predicted reactivity: Streptomyces viridochromogenes

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-BAR (36-50) antibodies

    From the left: 
    Arabidopsis thaliana wt - 1
    Arabidopsis thaliana overexpressing line 1 with BAR (BASTA) - 2
    Arabidopsis thaliana overexpressing line 1 with BAR (BASTA) - 3

    20 µg/well of total protein extracted freshly from Arabidopsis thaliana leaves with extraction buffer: 50 mM Tris/Cl pH 7,5 , 150 mM NaCl , 1% Nonidet P40 , 1 tablet Proteinase inhibitor cocktail/ 10 ml. and denatured with SDS sample buffer 95°C at °C were separated on 4-15% % SDS-PAGE  and blotted 1h to nitrocellulose (pore size of  0,45 µm), using wet transfer. Blot was blocked with 5 % milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for ON/4°C with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for 5 min. with Agrisera ECLBright (AS16 ECL-N-10). Exposure time was seconds.

    Courtesy of Dr. Dr. Birgit Kemmerling, ZMBP - Center for Plant Molecular Biology, Plant Biochemistry University of Tuebingen, Germany

  • Additional Information
  • Additional information (application): BAR (BASTA) gene is a selectable marker of plant genetic transformation, Nada (2016). Novel recombinant binary vectors harboring Basta (bar) gene as a plant selectable marker for genetic transformation of plants. Physiol Mol Biol Plants. 2016 Apr; 22(2): 241–251.
  • Background
  • Background: Phosphinothricin N-acetyltransferase (BAR) is an enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance, herbicide resitance gene. BAR (BASTA) gene is used as a selectable marker for genetic transformation of plants.

    Alternative names: PPT N-acetyltransferase, Phosphinothricin-resistance protein.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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