c-Myc tag, FITC conjugated (clone 9E10)

AS21 4627 | Clonality: Monoclonal | Host: Mouse | Reactivity: c-myc tagged proteins

Product Information

Immunogen Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and purified by size-exclusion chromatography.

Format Liquid at 1 mg/ml.

Quantity 0,1 mg, The conjugate is purified

Storage Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Flow cytometry (Flowcyt)

Recommended dilution 1-5 µg/ml (FlowCyt)

Reactivity

Confirmed reactivity c-Myc tagged fusion proteins

Application examples

Application examples Flow cytometry analysis using anti-c-Myc, FITC conjugated mouse monoclonal antibodies

Flow cytometry analysis of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (blue) using mouse monoclonal anti-c-Myc (9E10) FITC antibodies.

Flow cytometry analysis using anti-c-Myc tag FITC conjugated monoclonal antibodies

Flow cytometry analysis (intracellular staining) of transfected LST-1-c-Myc in HEK-293 cells (red-filled) compared with nontransfected HEK-293 cells (black-dashed) using mouse monoclonal anti-c-Myc (9E10) FITC conjugated antibodies.

Additional information

Background

Background c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.

Product citations

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